Accurate and objective infarct sizing by contrast-enhanced magnetic resonance imaging in a canine myocardial infarction model
Accurate and objective infarct sizing by contrast-enhanced magnetic resonance imaging in a canine myocardial infarction model
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DOI：
10.1016/j.jacc.2004.09.020
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发表时间：
2004-12-21
影响因子：
24
通讯作者：
Lima, JAC
中科院分区：
文献类型：
--
作者：
Amado, LC;Gerber, BL;Lima, JAC
为了确定一个准确的和可重复的方法来定义心肌梗死（MI）的大小，我们进行了一项研究，在一个闭胸犬模型的急性心肌梗死，其中MI的大小进行了测量，使用不同的阈值技术，并在造影剂administration.Background造影增强磁共振成像（CE-MRI）后，在不同的延迟时间成像。然而，以前的测量使用不均匀的方法，往往高估nonviable areas.METHODS十三只动物进行了90分钟的冠状动脉闭塞，然后再灌注。在再灌注后24 h内获得CE-MRI数据，并与氯化三苯基四氮唑病理学进行比较。在前9只动物中，使用反转恢复梯度回波脉冲序列，在钆二乙烯三胺五乙酸（Gd-DTPA）后15分钟获得类似的图像。为了确定最准确的方法，使用不同的标准，通过CE-MRI目视和半自动阈值技术测量MI尺寸。在另外四只动物中，每6分钟获取一次图像，直到Gd-DTPA.RESULTS后30分钟，死后MI大小为左心室容积的13.5 ± 2.6%。半自动技术，使用半峰全宽（FWHM）标准，与死后数据的相关性最好（r（2）= 0.94，p < 0.001; Bland-Altman图证实了结果）。使用FWHM，造影后不同延迟时间之间的MI大小没有差异（6和30 min分别为15.2 +/- 2.9%至14.5 +/- 4.2%; p = NS）。结论：当使用客观技术通过CE-MRI定义MI大小时，可以从造影剂给药后30 min获得的图像中获得准确的梗死大小测量值。(C)2004年，美国心脏病学会基金会。
OBJECTIVES To identify an accurate and reproducible method to define myocardial infarct (MI) size, we conducted a study in a closed-chest canine model of acute myocardial infarction, in which MI size was measured using different thresholding techniques and by imaging at different delay times after contrast administration.BACKGROUND The MI size by contrast-enhanced magnetic resonance imaging (CE-MRI) is directly related to long-term prognosis. However, previous measurements were done using nonuniform methods and tended to overestimate nonviable areas.METHODS Thirteen animals underwent 90 min of coronary artery occlusion, followed by reperfusion. The CE-MRI data were acquired within 24 h after reperfusion and compared with triphenyltetrazolium chloride pathology. In the first nine animals, images were obtained similar to15 min after gadolinium diethylene triamine penta-acetic acid (Gd-DTPA) using an inversion-recovery gradient-echo pulse sequence. To identify the most accurate method, MI size by CE-MRI was measured visually and by semi-automatic thresholding techniques, using different criteria. In four additional animals, images were acquired every 6 min until 30 min after Gd-DTPA.RESULTS Postmortem MI size was 13.5 +/- 2.6% of left ventricular volume. Semi-automatic techniques, using full-width at half-maximum (FWHM) criterion, correlated best with postmortem data (r(2) = 0.94, p < 0.001; results confirmed by Bland-Altman plots). Using FWHM, there was no difference in MI size between different delay times after contrast (15.2 +/- 2.9% to 14.5 +/- 4.2% at 6 and 30 min, respectively; p = NS).CONCLUSIONS When an objective technique is used to define MI size by CE-MRI, accurate infarct size measurements can be obtained from images obtained up to 30 min after contrast administration. (C) 2004 by the American College of Cardiology Foundation.