Rapid analysis method for paraquat and diquat in the serum using ion-pair high-performance liquid chromatography
Rapid analysis method for paraquat and diquat in the serum using ion-pair high-performance liquid chromatography
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DOI：
10.1248/bpb.28.725
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发表时间：
2005-04-01
影响因子：
2
通讯作者：
Yoshioka, T
中科院分区：
文献类型：
--
作者：
Ito, M;Hori, Y;Yoshioka, T
在本研究中，以日本东京GL Science Inc.的IPCC-MS3为流动相反离子，通过将去蛋白的血清样品直接引入高效液相色谱法，建立了一种在ODS柱上分离和定量百草枯和敌草枯的简单、快速的分析方法。在紫外290mN波长处测定百草枯和百草枯的线性关系良好，进样浓度在0.1~10.0mU g/m l范围内线性关系良好，检出限为0.05 mU g/m l，加标回收率分别为87.5%和89.1%，RSD分别为4.52%和3.85%。所有这些都是良好的结果，而且一次分析所需的时间不到30分钟。由于采用这种分析方法对4例急性中毒病例进行了分析，因此可以迅速决定目前所有病例的治疗方法。
In the present study, by using IPCC-MS3 (GL Sciences Inc. Tokyo, Japan) as the counter-ion in the mobile phase, we established a simple, quick method of analysis that separated and quantified paraquat and diquat on an ODS column by introducing the deproteinized serum sample directly into HPLC. The calibration curve of paraquat and cliquat detected at UV 290 mn showed good linearity when the concentration of the injected sample was in the range 0.1-10.0 mu g/ml. The detection limit was 0.05 mu g/ml, and the mean recoveries (n=5) added 1.0 mu g/ml each of paraquat and cliquat to standard serum were 87.5% and 89.1%, respectively, while the RSD were 4.52% and 3.85%. All of these were good results, and the time taken for one analysis was less than 30 min. As a result of employing this analytical method for the analyses in four cases of acute poisoning, it was possible to decide promptly on treatment approaches for all of the present cases.