Engineering of a Pluronic F127 functionalized magnetite/graphene nanohybrid for chemophototherapy
Engineering of a Pluronic F127 functionalized magnetite/graphene nanohybrid for chemophototherapy
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用于化学光疗的 Pluronic F127 功能化磁铁矿/石墨烯纳米杂化物的工程
DOI：
10.1088/0957-4484/25/6/065602
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发表时间：
2014-02-14
期刊：
影响因子：
3.5
通讯作者：
Hu, Haiqing
中科院分区：
文献类型：
--
作者：
Li, Yongyong;Liu, Jiaqiang;Hu, Haiqing
在这项研究中，一个多功能的石墨烯基纳米杂化物，被称为GN/Fe3O4/PF127，通过一个简单的一锅工艺设计，包括同时还原氧化石墨烯/Fe3O4，随后将Pluronic F127 （PF127）组装到石墨烯纳米片（GNs）上。GNs独特的芳香和平面结构允许附着多种功能成分，包括MRI造反差剂（Fe3O4纳米颗粒）和芳香抗癌药物如多柔比星（DOX），以及PF127涂层，赋予纳米杂化物生理分散性和稳定性。通过TEM观察、尺寸和FITR监测，揭示了成功的装配过程。与原始石墨烯或其氧化物衍生物相比，所得到的GN/Fe3O4/PF127纳米杂化物由于掺入了超顺磁性的Fe3O4纳米颗粒而没有明显的细胞毒性，因此具有较高的生物分散性和MRI诊断效果。此外，由于GN/Fe3O4/PF127纳米杂化材料在近红外区域具有相当大的光吸收，使得GN/Fe3O4/PF127纳米杂化材料具有光热效应。GN/Fe3O4/PF127纳米杂化物可能成为治疗性DOX辅助化学光疗的进一步平台。细胞毒性实验表明，负载dox的GN/Fe3O4/PF127纳米杂化物对HeLa细胞具有显著的细胞毒性，并且在光照射下细胞毒性作用增强。共聚焦激光扫描显微镜（CLSM）和流式细胞术分析（FCAS）表明，纳米杂化物很容易被HeLa细胞吸收。
In this study, a multifunctional graphene based nanohybrid, termed as GN/Fe3O4/PF127, is engineered via a facile one-pot process consisting of simultaneous reduction of graphene oxide/Fe3O4 and subsequent assembly of Pluronic F127 (PF127) onto graphene nanosheets (GNs). The unique aromatic and planar structure of GNs allows the attachment of multiple functional components including MRI contrast agent (Fe3O4 nanoparticles) and an aromatic anticancer drug like doxorubicin (DOX), as well as PF127 coating which imparts physiological dispersivity and stability to the nanohybrid. The successful assembly process is revealed by TEM observation, size and FITR monitoring. In contrast with the primitive graphene or its oxide derivative, the resulting GN/Fe3O4/PF127 nanohybrids have shown high biological dispersion and MRI effect for diagnosis due to the incorporation of superparamagnetic Fe3O4 nanoparticles without evident cytotoxicity. Moreover, the GN/Fe3O4/PF127 nanohybrid exhibits a photothermal effect due to the considerable optical absorption in the near-infrared region of GNs. The GN/Fe3O4/PF127 nanohybrid could be a further platform for chemophototherapy assisted by the therapeutic DOX. Cellular toxicity assays indicated that the DOX-loaded GN/Fe3O4/PF127 nanohybrid showed a remarkable cytotoxicity to HeLa cells and the cytotoxic effect was intensified when subjected to photoirradiation. Confocal laser scanning microscopy (CLSM) and flow cytometric analysis (FCAS) revealed that the nanohybrid could be easily uptaken into HeLa cells.