Establishment and characterization of chronic-hypoxia-resistant gastric cancer cell line MNK45/HYP
Establishment and characterization of chronic-hypoxia-resistant gastric cancer cell line MNK45/HYP
复制标题
耐慢性缺氧胃癌细胞系MNK45/HYP的建立及鉴定
DOI：
10.1007/s11596-011-0149-1
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hui
中科院分区：
文献类型：
--
作者：
H. Qiu;Guangyuan Hu;Hui
建立耐慢性缺氧的胃癌细胞系，探讨其生物学特性，并与亲代细胞系进行比较。胃癌细胞系在降氧培养条件下培养。用细胞计数法计算细胞倍增时间。用四甲基偶氮唑盐比色法检测细胞的耐药能力。集落形成法测定细胞的辐射耐受性。流式细胞仪检测细胞周期分布。用Transwell法检测其侵袭能力。用免疫印迹法检测GLUT-1和HIF-1α的表达水平。MNK45/HYP细胞在1%氧气浓度下存活，倍增时间为35.01±1.02h，MNK45细胞倍增时间为27.35±0.83h(P<；0.01)。MNK45/HYP细胞处于G0/G1期比例为(58.3±6.1)%，MNK45细胞比例为(42.2±6.0)%(P<0.05)。与亲本细胞MNK45相比，5-FU、PTX、OXA、SN38、GEM和VP16的耐药指数分别为5.3、1.3、3.6、2.2、4.8和4.4。照射后MNK45/HYP细胞的集落形成能力也明显高于MNK45细胞。MNK45/HYP细胞的侵袭数为107.7±17.5，MNK45细胞的侵袭数为59.0±9.9。GLUT-1和HIF-1α在MNK45/HYP细胞中的表达水平明显高于MNK45细胞。MNK45/HYP细胞具有低氧肿瘤的生物学特性，对慢性低氧具有良好的耐受性，可用于慢性低氧条件下实体瘤的研究。
SummaryThe chronic-hypoxia-resistant gastric cancer cell line was established, and its biological characteristics were explored and compared with the parental cell line. Gastric cancer cell lines were cultured under the degressive oxygen concentration. Cell doubling time was calculated by cell counting method. Chemo-resistance ability of cells was tested by MTT assay. Irradiation tolerance of cells was evaluated by colony forming method. Cell cycle distribution was tested with flow cytometry. Invasive ability was tested by Transwell method. The expression levels of GLUT-1 and HIF-1α were detected by using Western blot. MNK45/HYP cells successfully survived under the 1% concentration of oxygen and its cell doubling time was 35.01±1.02 h, while that of MNK45 was 27.35±0.83 h (P<0.01). The percentage of MNK45/HYP cells in G0/G1 stage was (58.3±6.1)%, and that of MNK45 cells was (42.2±6.0)% (P<0.05). Comparing with the parental cells MNK45, drug resistance indexes of 5-Fu, PTX, OXA, Sn38, GEM and VP16 in MNK45/HYP cells were respectively 5.3, 1.3, 3.6, 2.2, 4.8 and 4.4. Colony forming ability of MNK45/HYP cells after irradiation was also significantly higher than MNK45 cells. The invasive number of MNK45/HYP cells was 107.7±17.5, while that of MNK45 cells was 59.0±9.9. The expression levels of GLUT-1 and HIF-1α in MNK45/HYP cells were significantly higher than those in MNK45 cells. MNK45/HYP cells hold biological characteristics of hypoxia tumor with good tolerance to chronic hypoxia, and can be used for the research of solid tumor under chronic hypoxia condition.
影响因子：
5.8
作者：
Mahoney, BP;Raghunand, N;Gillies, RJ
通讯作者：
Gillies, RJ
影响因子：
4.8
作者：
Gardner, LB;Li, Q;Dang, CV
通讯作者：
Dang, CV