Mechanism of estrogen action: indirect effect of estradiol-17 beta on proliferation of quail oviduct cells.
Mechanism of estrogen action: indirect effect of estradiol-17 beta on proliferation of quail oviduct cells.
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雌激素作用机制：雌二醇17β对鹌鹑输卵管细胞增殖的间接影响。
DOI：
10.1073/pnas.80.6.1621
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发表时间：
1983
影响因子：
11.1
通讯作者：
Sonnenschein,C
中科院分区：
文献类型：
--
作者：
Laugier,C;Pageaux,JF;Soto,AM;Sonnenschein,C
收集实验数据以测试雌激素的作用是否需要类固醇直接作用于其靶细胞以诱导(i)细胞增殖和（ii）细胞类型特异性蛋白质合成。取去卵巢的鹌鹑经颈静脉或门静脉灌注不同剂量雌二醇-17β (E2) (0.05 ~ 6.8 ng/min) 24小时。当颈静脉灌注速率大于0.5 ng/min时，e2血药浓度逐渐升高。而在门静脉中，只有当灌注速率大于2 ng/min时，e2血浆浓度才较未灌注去卵巢动物升高。无论给药途径和灌注速率如何，从0.5 ng/min开始，观察到每条输卵管DNA浓度的增加。当e2以0.5 ng/min或更高的流速通过颈静脉灌注时，核嗜雌激素增加。即使在2.0 ng/min的灌注速率下，经门静脉灌注的鹌鹑输卵管也没有增加相同的参数。以2 ng/min及以上的流速经颈静脉灌注鹌鹑输卵管诱导黄体促孕素；另一方面，通过门静脉灌注2 ng/min以上的促孕素诱导了鸟类。在任何灌注速率和途径下，鹌鹑输卵管均未产生卵清蛋白。然而，诱导细胞类型特异性蛋白（孕激素）的合成似乎需要E2的直接作用。e2浓度对促孕素诱导的影响大于对输卵管细胞增殖的影响。这些结果表明e2对细胞增殖的影响是间接的，它涉及肝脏的中间步骤，并且不需要增加核嗜雌激素蛋白的浓度。
Experimental data were collected to test whether the effect of estrogens required a direct action of the steroid on their target cells for induction of (i) cell multiplication and (ii) cell-type-specific protein synthesis. Ovariectomized quails were perfused for 24 hr with several doses of estradiol-17β (E2) (0.05-6.8 ng/min) through either the jugular vein or the portal vein. E2plasma concentrations increased progressively when the perfusion rate through the jugular vein was 0.5 ng/min and higher. With the portal vein, by contrast, E2plasma concentrations increased over the concentration in unperfused ovariectomized animals only when the perfusion rate was above 2 ng/min. An increase in DNA concentration per oviduct was observed regardless of the route of administration and the rate of perfusion, starting at 0.5 ng/min. Nuclear estrophilins increased when E2was perfused through the jugular vein at rates of 0.5 ng/min or greater. This same parameter was not increased in oviducts of quail perfused through the portal vein even at a perfusion rate of 2.0 ng/min. Progestophilins were induced in the oviducts of quail perfused through the jugular vein at rates of 2 ng/min and above; on the other hand, progestophilins were induced in birds perfused through the portal vein at rates above 2 ng/min. Ovalbumin was not induced in quail oviducts at any rate and route of perfusion. The induction of the synthesis of cell-type-specific protein (progestophilins, in this case) seems to require, however, the direct action of E2. The E2concentrations effecting the induction of progestophilins were higher than those necessary to effect the proliferation of oviduct cells. These results suggest that the E2effect on cell proliferation is indirect, it involves an intermediary step at the liver, and it does not require increased concentration of nuclear estrophilins.