TNF mediates lung leak, but not neutrophil accumulation, in lungs of rats given IL-1 intratracheally.
TNF mediates lung leak, but not neutrophil accumulation, in lungs of rats given IL-1 intratracheally.
复制标题
在气管内给予 IL-1 的大鼠肺部，TNF 介导肺渗漏，但不介导中性粒细胞积聚。
DOI：
10.1164/ajrccm.155.6.9196104
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Repine,JE
中科院分区：
文献类型：
--
作者：
Hybertson,BM;Jepson,EK;Cho,OJ;Clarke,JH;Lee,YM;Repine,JE
从急性呼吸窘迫综合征患者的肺灌洗液中，白细胞介素1(IL-1)增加，大鼠气管内给予IL-1会导致急性、中性粒细胞依赖的氧化性肺漏。我们发现，气管内注射IL-1的大鼠肺灌洗液肿瘤坏死因子(TNF)水平增加，静脉注射肿瘤坏死因子结合蛋白(TNFBP)的大鼠不会出现像气管内注射IL-1后发生的肺渗漏增加。相反，气管内注射IL-1和静脉注射TNFBP的大鼠肺灌洗液中性粒细胞聚集和肺灌洗液细胞因子诱导的中性粒细胞趋化因子水平与气管内注射IL-1的大鼠相同。我们的结果表明，在大鼠气管内注射IL-1后，TNFBP减少了中性粒细胞介导的肺渗漏，但不能减少中性粒细胞在肺内的聚集。
Interleukin-1 (IL-1) is increased in lung lavages obtained from patients with acute respiratory distress syndrome, and administering IL-1 intratracheally to rats causes an acute, neutrophil-dependent, oxidative lung leak. We found that rats given IL-1 intratracheally had increased lung lavage fluid tumor necrosis factor (TNF) levels, and that rats treated with TNF binding protein (TNFbp) intravenously did not develop the increased lung leak that occurs after administration of IL-1 intratracheally. In contrast, rats given IL-1 intratracheally and TNFbp intravenously had the same elevations in lung lavage neutrophil accumulation and lung lavage cytokine-induced neutrophil chemoattractant levels as rats given IL-1 intratracheally. Our results show that TNFbp decreases neutrophil-mediated lung leak, but not lung neutrophil accumulation, after administration of IL-1 intratracheally in rats.