In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)
In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)
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DOI：
10.1007/s00436-010-2211-5
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发表时间：
2011-06-01
影响因子：
2
通讯作者：
Wang, Gao-Xue
中科院分区：
文献类型：
--
作者：
Wu, Zong-Fan;Zhu, Bin;Wang, Gao-Xue
本研究对苦杏仁（Prunus amygdalus Batsch）、升麻（Cimicifuga foetida L.）根茎（Rhizoma Cimicifugae）、紫花前胡Peucedanum decursivum（Miq.）Maxim roots（Radix Peucedani），Momelia cochinchinensis Spreng.种子（木麻黄种子）和柴胡（Bupleurumchinense DC.）根（柴胡）对金鱼体内的中间指环虫（Dactylogyrus intermedius）具有体内驱虫活性。结果表明，红毛菊甲醇、氯仿、乙酸乙酯提取物对红毛菊的药效最强。结果表明，柴胡提取物的48 h-EC（50）和EC（90）值分别为3.5和6.9、6.0和8.4、7.4和11.2mg/L，其次是杜鹃花乙酸乙酯提取物;升麻和R.白花前胡EC（50）分别为189.2和240.4mg/L。采用甲醇、氯仿和乙酸乙酯提取物对红毛菊进行了初步的研究。为评价其对寄主的安全性，进行了中华柴胡的急性毒性试验。暴露48小时后，金鱼的死亡率被记录下来，建立的LC（50）值分别比相应的EC（50）高10.1倍，4.2倍和8.4倍。这些结果表明，从R.北柴胡具有作为天然抗寄生虫药物防治棉铃虫的潜力。中间体，尤其是甲醇。
In the present study, an attempt has been made to petroleum ether, chloroform, ethyl acetate, methanol, and water extracts of Prunus amygdalus Batsch seeds (Semen amygdali), Cimicifuga foetida L. rhizomes (Rhizoma Cimicifugae), Peucedanum decursivum (Miq.) Maxim roots (Radix Peucedani), Momordica cochinchinensis Spreng. seeds (Semen Momordicae), and Bupleurum chinense DC. roots (Radix Bupleuri chinensis) for their in vivo anthelmintic activity against monogenean Dactylogyrus intermedius in goldfish (Carassius auratus). The results showed that the efficacies of methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were found to be, in this order, more effective than others with the 48 h-EC(50) and EC(90) values of 3.5 and 6.9, 6.0 and 8.4, 7.4 and 11.2 mg/L, respectively, followed by ethyl acetate extract of R. cimicifugae and chloroform extract of R. peucedani with EC(50) 189.2 and 240.4 mg/L. The promising methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were subjected to acute toxicity tests for the evaluation of their safety to the host. After 48-h exposure, the mortalities of goldfish were recorded, and the established LC(50) values were 10.1-, 4.2-, and 8.4-fold higher than the corresponding EC(50). These results indicated that the three extracts from R. Bupleuri chinensis exhibit potential to be used as preferred natural antiparasitics for the control of the D. intermedius, especially for the methanolic one.