Study of sequence variation of dengue type 3 virus in naturally infected mosquitoes and human hosts: Implications for transmission and evolution
Study of sequence variation of dengue type 3 virus in naturally infected mosquitoes and human hosts: Implications for transmission and evolution
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DOI：
10.1128/jvi.78.22.12717-12721.2004
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发表时间：
2004-11-01
影响因子：
5.4
通讯作者：
Wang, WK
中科院分区：
文献类型：
--
作者：
Lin, SR;Hsieh, SC;Wang, WK
登革热病毒是一种在蚊子媒介和人类宿主中交替复制的虫媒病毒。我们研究了自然感染埃及伊蚊和同一疫情中8名患者的3型登革热病毒序列，报告称蚊子的序列变异程度一般低于患者(包膜基因[E]和衣壳基因[C]的平均多样性分别为0.21比0.38%和0.09比0.23%)。5只实验感染的蚊子进一步证实了这一点(E和C基因的平均多样性分别为0.09%和0.10%)。对蚊子和病人的E序列的准种结构的检查表明，主要变异的序列是相同的，表明主要变异是传播的。这些发现支持我们的假设，即蚊子通过在传播过程中保持更同质的病毒种群和一个显性变异，为登革热病毒的进化保护做出了贡献。
Dengue virus is an arbovirus that replicates alternately in the mosquito vector and human host. We investigated sequences of dengue type 3 virus in naturally infected Aedes aegypti mosquitoes and in eight patients from the same outbreak and reported that the extent of sequence variation seen with the mosquitoes was generally lower than that seen with the patients (mean diversity, 0.21 versus 0.38% and 0.09 versus 0.23% for the envelope [E] and capsid [C] genes, respectively). This was further verified with five experimentally infected mosquitoes (mean diversity, 0.09 and 0.10% for the E and C genes, respectively). Examination of the quasispecies structures of the E sequences of the mosquitoes and of the patients revealed that the sequences of the major variants were the same, suggesting that the major variant was transmitted. These findings support our hypothesis that mosquitoes contribute to the evolutionary conservation of dengue virus by maintaining a more homogenous viral population and a dominant variant during transmission.