Main pancreatic duct, common bile duct and sphincter of Oddi structure visualized by optical coherence tomography: An ex vivo study compared with histology
Main pancreatic duct, common bile duct and sphincter of Oddi structure visualized by optical coherence tomography: An ex vivo study compared with histology
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DOI：
10.1016/j.dld.2006.02.014
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发表时间：
2006-06-01
影响因子：
4.5
通讯作者：
Doglioni, C.
中科院分区：
文献类型：
--
作者：
Testoni, P. A.;Mariani, A.;Doglioni, C.
背景已经提出光学相干断层扫描以获得胃肠道组织结构的高分辨率成像。胆总管、主胰管和Oddi括约肌壁结构的光学相干断层扫描（OCT）表现至今尚未明确。在一项前瞻性研究中，比较胰胆管系统的光学相干断层扫描图像与组织学，并确定正常管壁结构的光学相干断层扫描模式。在切除后1小时内，通过光学相干断层扫描对5个连续离体人胰腺标本的非肿瘤节段的多个切片进行了研究。将60例光学相干断层扫描图像与相应的组织学结果进行比较。正常胆总管、主胰管和Oddi括约肌的光学相干断层扫描外观的特征在于具有规则表面和均匀背散射信号的分化的三层结构，分别对应于单层上皮细胞、结缔-肌肉层和肌肉或腺泡结构。所有病例的光学相干断层扫描和组织学检查结果一致。光学相干断层扫描能够提供正常胆总管，主胰管和Oddi括约肌的壁结构的实时图像，这些图像与通过组织学获得的图像相似。这些结果表明，光学相干断层扫描可以使高分辨率图像从胰胆系统在ERCP程序中获得。(c)2006年意大利胃肠病学编辑由爱思唯尔有限公司出版。保留所有权利。
Background. Optical coherence tomography has been proposed to obtain high-resolution imaging of tissue structure of GI tract. Up till now, the optical coherence tomography appearance of the common bile duct, main pancreatic duct and sphincter of Oddi wall structure has not yet been defined.Aims. To compare, in a prospective study, optical coherence tomography images of pancreato-biliary ductal system with histology and identify the optical coherence tomography pattern of the normal wall structure of the ducts.Methods. Multiple sections of non-neoplastic segments of five consecutive ex vivo human pancreatic specimens were investigated by optical coherence tomography scanning within 1 h of resection. Sixty optical coherence tomography images were compared with the corresponding histological findings.Results. Optical coherence tomography appearance of normal common bile duct, main pancreatic duct and sphincter of Oddi is characterized by a differentiated three-layer architecture with a regular surface and a homogeneous back-scattered signal, corresponding to the single layer of epithelial cells, the connective-muscular layer and the muscular or acinar structure, respectively. Optical coherence tomography and histology findings were concordant in all cases.Conclusions. Optical coherence tomography was able to provide in real-time images of wall structure of the normal common bile duct, main pancreatic duct and sphincter of Oddi that are similar to those obtained by histology. These results suggest that optical coherence tomography could enable high-resolution images to be obtained from the pancreato-biliary system during an ERCP procedure. (c) 2006 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.