Reduction of spinning sidebands in proton NMR of human prostate tissue with slow high-resolution magic angle spinning
Reduction of spinning sidebands in proton NMR of human prostate tissue with slow high-resolution magic angle spinning
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DOI：
10.1002/mrm.20523
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发表时间：
2005-07-01
影响因子：
3.3
通讯作者：
Cheng, LL
中科院分区：
文献类型：
--
作者：
Burns, MA;Taylor, JL;Cheng, LL
高分辨率魔角旋转(HRMAS)核磁共振波谱已被证明是有用的，分析完整的组织，并允许在组织代谢物和疾病病理之间建立关联。将这些研究扩展到慢速旋转方法，有助于保护组织病理结构免受HRMAS离心机的破坏，并可能允许对更大对象的研究。由慢速旋转产生的旋转边带(SSB)必须被抑制，以防止代谢光谱区的复杂化。在这项研究中，人的前列腺组织以及代谢物混合物溶液的凝胶样品在14.1T光谱仪上用连续波(CW)水预饱和测量，HRMAS的旋转速度分别为250、300、350、600、700 Hz和3.0 kHz。通过简单的最小函数(对于在不同但接近的自旋速度下获得的N光谱的Min(A，B，...，N))编辑光谱产生无SSB的光谱。在低自转速度下产生的Min(A，B，...，N)编辑谱与从3 kHz谱测量的浓度以及与定量病理学的定量结果之间存在统计上显著的线性相关。这些结果表明，这一方案在分析完整组织方面具有经验性的实用价值，也可以作为病理学诊断疾病的辅助工具。(C)2005年Wiley-Liss，Inc.
High-resolution magic angle spinning (HRMAS) NMR spectroscopy has proven useful for analyzing intact tissue and permitting correlations to be made between tissue metabolites and disease pathologies. Extending these studies to slow-spinning methodologies helps protect tissue pathological structures from HRMAS centrifuging damage and may permit the study of larger objects. Spinning sidebands (SSBs), which are produced by slow spinning, must be suppressed to prevent the complication of metabolic spectral regions. In this study human prostate tissues, as well as gel samples of a metabolite mixture solution, were measured with continuous-wave (CW) water pre-saturation on a 14.1T spectrometer, with HRMAS spinning rates of 250, 300, 350, 600, and 700 Hz, and 3.0 kHz. Editing the spectra by means of a simple minimum function (Min(A, B,..., N) for N spectra acquired at different but close spinning rates) produced SSB-free spectra. Statistically significant linear correlations were observed for metabolite concentrations quantified from the Min(A, B,..., N)-edited spectra generated at low spinning rates, with concentrations measured from the 3 kHz spectra, and also with quantitative pathology. These results indicate the empirical utility of this scheme for analyzing intact tissue, which also may be used as an adjunct tool in pathology for diagnosing disease. (c) 2005 Wiley-Liss, Inc.