Leaf Growth Response to Mild Drought: Natural Variation in Arabidopsis Sheds Light on Trait Architecture
Leaf Growth Response to Mild Drought: Natural Variation in Arabidopsis Sheds Light on Trait Architecture
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DOI：
10.1105/tpc.16.00483
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发表时间：
2016-10-01
期刊：
影响因子：
11.6
通讯作者：
Inze, Dirk
中科院分区：
文献类型：
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作者：
Clauw, Pieter;Coppens, Frederik;Inze, Dirk
可用水量的减少会对植物生长和作物产量产生负面影响。然而，人们对非致命性干旱条件下的生长调控机制缺乏清晰的认识。基因组学、表型组学和转录组学的最新进展允许对自然变异进行深入分析。在这项研究中，我们对全球范围内的拟南芥种质的叶子生长反应进行了详细的筛选。通过对不同的叶片生长表型进行全基因组关联图谱分析并表征年轻发育叶片的转录组，研究了轻度干旱时生长反应的遗传结构。尽管没有发现主要效应位点与轻度干旱中的生长相关，但转录组分析进一步深入了解了特定组织中对轻度干旱的转录反应的自然变化。共表达分析表明存在在不同遗传背景下共变的基因簇，其中包括在渗透胁迫的生长反应中具有重要调节功能的基因簇。研究发现，根据 283 个基因的表达谱，可以高精度地推断出叶片中是否发生了轻度干旱胁迫反应。对表达数据的全基因组关联研究表明，在对环境扰动的转录反应中，反式调节似乎比顺式调节更重要。
Plant growth and crop yield are negatively affected by a reduction in water availability. However, a clear understanding of how growth is regulated under nonlethal drought conditions is lacking. Recent advances in genomics, phenomics, and transcriptomics allow in-depth analysis of natural variation. In this study, we conducted a detailed screening of leaf growth responses to mild drought in a worldwide collection of Arabidopsis thaliana accessions. The genetic architecture of the growth responses upon mild drought was investigated by subjecting the different leaf growth phenotypes to genome-wide association mapping and by characterizing the transcriptome of young developing leaves. Although no major effect locus was found to be associated with growth in mild drought, the transcriptome analysis delivered further insight into the natural variation of transcriptional responses to mild drought in a specific tissue. Coexpression analysis indicated the presence of gene clusters that co-vary over different genetic backgrounds, among others a cluster of genes with important regulatory functions in the growth response to osmotic stress. It was found that the occurrence of a mild drought stress response in leaves can be inferred with high accuracy across accessions based on the expression profile of 283 genes. A genome-wide association study on the expression data revealed that trans regulation seems to be more important than cis regulation in the transcriptional response to environmental perturbations.