Expression of Th1 and Th2 cytokine-associated transcription factors, T-bet and GATA-3, in the eutopic endometrium of women with endometriosis
Expression of Th1 and Th2 cytokine-associated transcription factors, T-bet and GATA-3, in the eutopic endometrium of women with endometriosis
复制标题
子宫内膜异位症女性在位子宫内膜中 Th1 和 Th2 细胞因子相关转录因子 T-bet 和 GATA-3 的表达
DOI：
10.1016/j.acthis.2012.01.005
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Wang, Danbo
中科院分区：
文献类型：
--
作者：
Chen, Peng;Zhang, Zhifang;Wang, Danbo
本研究旨在探讨T细胞表达的T-box(T-bet)和GATA结合蛋白3(GATA-3)在子宫内膜异位症患者在位内膜中的表达。子宫内膜组织采集自20例经腹腔镜确诊的子宫内膜异位症患者和20例非子宫内膜异位症患者。采用实时定量聚合酶链式反应(QPCR)、免疫组织化学和Western印迹分析检测T-bet和GATA-3的表达。子宫内膜异位症患者在位内膜组织中T-bet mRNA表达水平较低，而GATA-3 mRNA表达水平较高，导致T-bet/GATA-3 mRNA比值显著降低(P<；0.05)。Western印迹分析显示，子宫内膜异位症组T-bet/GATA-3蛋白比值也显著低于对照组(P<；0.05)。提示T-bet和GATA-3可能是细胞因子调控基因，而Th2特异性转录因子GATA-3可能在免疫应答和子宫内膜异位症的发生发展中起重要作用。(C)2012年爱思唯尔股份有限公司。版权所有。
The objective of the present study was to evaluate the expression of T-box expressed in T cells (T-bet) and GATA binding protein 3 (GATA-3) in the eutopic endometrium from women with endometriosis. Endometrial tissues were collected from 20 women with laparoscopically confirmed endometriosis and 20 women without endometriosis. T-bet and GATA-3 expression was measured by quantitative real-time PCR (qPCR), immunohistochemistry and Western blot analysis. Eutopic endometrial tissues from patients with endometriosis expressed lower levels of T-bet mRNA and high levels of GATA-3 mRNA, leading to a significant lower T-bet/GATA-3 mRNA ratio (P < 0.05). Western blot analysis showed that the T-bet/GATA-3 protein ratio in endometriosis group was also statistically lower than that in the control group (P < 0.05). These results suggested that T-bet and GATA-3 may act as cytokine regulatory genes, and the Th2-specific transcription factor, GATA-3, probably plays an essential role in the immune response and the development of endometriosis. (c) 2012 Elsevier GmbH. All rights reserved.