The influence of light-curing time on the bacterial colonization of resin composite surfaces
The influence of light-curing time on the bacterial colonization of resin composite surfaces
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DOI：
10.1016/j.dental.2009.02.012
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发表时间：
2009-09-01
期刊：
影响因子：
5
通讯作者：
Garcia-Godoy, Franklin
中科院分区：
文献类型：
--
作者：
Brambilla, Eugenio;Gagliani, Massimo;Garcia-Godoy, Franklin
目标。复合材料表面的细菌定植是粘接剂修复体周围继发性龋齿的主要病因。研究了光固化时间对树脂复合材料中变形链球菌定植的影响。从牙科树脂复合材料中获得的样品分为12组，分别在相同光源下使用400和800 mW/cm两种不同的固化功率进行10、20、30、40、60或80 s的光固化(2)。分离出一株MS野生菌株，获得附着在样品表面的24小时单特异性生物膜。基于将黄色四氮唑盐还原为紫色甲酸盐的比色技术（MTT测定）用于评估粘附在样品表面的生物量。采用方差分析和Schefffe检验对结果进行统计分析。双因素方差分析显示，固化时间因子与固化功率因子之间无交互作用（p = 0.970）；采用单因素方差分析分别对各固化功率水平的数据进行分析。两个水平在不同时间组间差异极显著（p < 0.0001）。趋势的非参数检验显示，随着固化时间的增加，两个水平上细菌定植减少的趋势都非常显著（p < 0.0001）。减少固化时间似乎是负责增加体外定植复合表面的质谱；这种现象可能与材料表面存在未聚合的单体有关。(C) 2009年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objectives. Bacterial colonization of composite surfaces represents the main factor in the etiology of secondary caries around adhesive restorations. The authors' aim was to evaluate the influence of light-curing time on mutans streptococci colonization (MS) of a resin composite material.Methods. Specimens obtained from a dental resin composite were divided into 12 groups and light-cured with the same light source respectively for 10, 20, 30, 40, 60 or 80 s using two different curing-power levels: 400 and 800 mW/cm(2). A wild strain of MS was isolated and a 24-h-monospecific biofilm, adherent to the surfaces of the samples, was obtained. A colorimetric technique (MTT assay), based on the reduction of a yellow tetrazolium salt to a purple formazan, was used to evaluate the biomass adherent to the specimen surfaces. ANOVA and Schefffe's tests were used to statistically analyze the results.Results. Two-way ANOVA demonstrated there was no interaction between curing-time factor and curing-power factor (p = 0.970); one-way ANOVA was used to analyze separately the data obtained from each curing-power level. Both levels showed highly significant differences (p < 0.0001) among the different curing time groups. The non-parametric test for trend showed in both levels the existence of a highly significant trend (p < 0.0001) for bacterial colonization reduction as curing time increases.Significance. A reduced curing time seems to be responsible for increased in vitro colonization of composite surfaces by MS; this phenomenon is likely to be related to the presence of unpolymerized monomers on the material surface. (C) 2009 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.