Gene expression throughout a vertebrate's embryogenesis
Gene expression throughout a vertebrate's embryogenesis
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DOI：
10.1186/1471-2164-12-132
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发表时间：
2011-02-28
期刊：
影响因子：
4.4
通讯作者：
Oleksiak, Marjorie F.
中科院分区：
文献类型：
--
作者：
Bozinovic, Goran;Sit, Tim L.;Oleksiak, Marjorie F.
背景资料：描述胚胎发育过程中的基因表达模式拓宽了我们对定义形态发生的过程和模式的理解。然而，在整个脊椎动物胚胎发生过程中，基因表达模式尚未被描述。本研究提出了统计分析的基因表达在所有40个发展阶段的硬骨鱼底heteroclitus使用四个生物学重复stage.Results：模式的基因表达的7,000个基因似乎是重要的，因为他们概括的发展时间。在45%的基因之间具有显着的表达差异对时间上相邻的阶段，显着差异的基因表达变化少至5个以上的660。五个相邻的阶段相对于其他阶段具有不成比例的更显著的基因表达变化（> 200个基因）：4至8和8至16个细胞阶段、循环开始、孵化前和孵化后以及完全卵黄吸收期间。在原肠胚形成期间，相邻阶段之间的差异最小。然而，在第16阶段（前中期原肠胚形成），最大数量的基因具有峰值表达。这个阶段在氧化呼吸和蛋白质表达（核糖体、翻译基因和蛋白酶）中具有过度表达的基因。出乎意料的是，在所有核糖体基因中，既存在强正相关性，也存在强负相关性。相似的相关模式的表达发生在所有显着的genes.Conclusions：这些数据提供了统计支持的时间动态的发育基因表达在脊椎动物发育的各个阶段。
Background: Describing the patterns of gene expression during embryonic development has broadened our understanding of the processes and patterns that define morphogenesis. Yet gene expression patterns have not been described throughout vertebrate embryogenesis. This study presents statistical analyses of gene expression during all 40 developmental stages in the teleost Fundulus heteroclitus using four biological replicates per stage.Results: Patterns of gene expression for 7,000 genes appear to be important as they recapitulate developmental timing. Among the 45% of genes with significant expression differences between pairs of temporally adjacent stages, significant differences in gene expression vary from as few as five to more than 660. Five adjacent stages have disproportionately more significant changes in gene expression (> 200 genes) relative to other stages: four to eight and eight to sixteen cell stages, onset of circulation, pre and post-hatch, and during complete yolk absorption. The fewest differences among adjacent stages occur during gastrulation. Yet, at stage 16, (pre-midgastrulation) the largest number of genes has peak expression. This stage has an over representation of genes in oxidative respiration and protein expression (ribosomes, translational genes and proteases). Unexpectedly, among all ribosomal genes, both strong positive and negative correlations occur. Similar correlated patterns of expression occur among all significant genes.Conclusions: These data provide statistical support for the temporal dynamics of developmental gene expression during all stages of vertebrate development.