In vivo optical imaging of physiological responses to photostimulation in human photoreceptors
In vivo optical imaging of physiological responses to photostimulation in human photoreceptors
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DOI：
10.1073/pnas.1606428113
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发表时间：
2016-11-15
影响因子：
11.1
通讯作者：
Huettmann, Gereon
中科院分区：
文献类型：
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作者：
Hillmann, Dierck;Spahr, Hendrik;Huettmann, Gereon
视觉分子和细胞过程的无创功能成像可能对研究和临床诊断产生巨大影响。虽然已经在离体和固定动物体内观察到合适的本征光信号，但在活体中检测光感受器活性的本征光信号是繁琐且耗时的。在这里，我们观察到光感受器外段的光路长度在空间和时间上发生了清晰的变化，这是对人眼光刺激的响应。为了见证这些变化，我们评估了用并行化和计算像差校正的光学相干层析成像系统获得的相位数据。光路长度变化的无创检测显示了活体人类视网膜中单个视锥细胞的神经元光感受器活性，因此，它可能为眼科和神经病学提供诊断选择，并可能为人类视觉光传导提供见解。
Noninvasive functional imaging of molecular and cellular processes of vision may have immense impact on research and clinical diagnostics. Although suitable intrinsic optical signals (IOSs) have been observed ex vivo and in immobilized animals in vivo, detecting IOSs of photoreceptor activity in living humans was cumbersome and time consuming. Here, we observed clear spatially and temporally resolved changes in the optical path length of the photoreceptor outer segment as a response to an optical stimulus in the living human eye. To witness these changes, we evaluated phase data obtained with a parallelized and computationally aberration-corrected optical coherence tomography system. The noninvasive detection of optical path length changes shows neuronal photoreceptor activity of single cones in living human retina, and therefore, it may provide diagnostic options in ophthalmology and neurology and could provide insights into visual phototransduction in humans.