Enterocyte dynamics and mucosal development in the posthatch chick
Enterocyte dynamics and mucosal development in the posthatch chick
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DOI：
10.1093/ps/80.6.776
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发表时间：
2001-06-01
期刊：
影响因子：
4.4
通讯作者：
Sklan, D
中科院分区：
文献类型：
--
作者：
Geyra, A;Uni, Z;Sklan, D
本研究观察了雏鸡孵化后12 d内小肠粘膜形态学和肠细胞动力学的变化。在孵化，肠上皮细胞是圆形和非极性的，然而，在24小时posthatch，肠上皮细胞延长，表现出更典型的形态。地穴在孵化和48小时内陷基本完成，地穴数量增加的分支和分裂，与地穴每绒毛达到高原72小时后，posthatch。所有上皮细胞在孵化时都是增殖的。在隐窝中，增殖的肠细胞的比例下降到50 - 60%，而沿着绒毛的增殖细胞的比例下降到10 - 20%，由6天后，在十二指肠，空肠和回肠中观察到不同的绒毛的时间发育模式。在整个实验过程中，十二指肠中的绒毛表面积稳步增加，而单个空肠和回肠绒毛表面积在4 d posthatch后增加较慢。在十二指肠和空肠，但不是在回肠肠横截面的绒毛数量增加。各肠段绒毛总表面积的增加趋势基本一致，至孵化后3d，空肠的吸收面积显著增加，而十二指肠和回肠的吸收面积增加较慢。这项研究表明，在孵化小鸡，小肠成熟的方式类似于新生哺乳动物，具体的个体发育时间表在不同的小肠段，然而，最显着的变化发生在第一个24小时posthatch。
Changes in the morphology of the small intestinal mucosa and enterocyte dynamics were examined in posthatch chicks through 12 d. At hatch, enterocytes were round and nonpolar; however, within 24 h posthatch, enterocytes lengthened and exhibited more typical morphology. Crypts were rudimentary at hatch and by 48 h invagination was completed and crypt numbers increased by branching and fission, with the number of crypts per villus reaching plateau after 72 h posthatch. All epithelial cells were proliferative at hatch. In the crypts, the proportion of proliferating enterocytes decreased to 50 to 60% within 2 d posthatch, whereas along the villus the proportion of proliferating cells decreased to 10 to 20% by 6 d.Different patterns of temporal development of villi were observed in the duodenum, jejunum and ileum. Individual villus surface area increased steadily in the duodenum throughout the experiment, whereas individual jejunal and ileal villus surface areas increased more slowly after 4 d posthatch. The number of villi per cross-section of intestine increased in the duodenum and jejunum but not in the ileum. The total segment villus surface area increased similarly in all segments until 3 d posthatch, after which the jejunum increased considerably in absorptive area, whereas the duodenum and ileum increased more slowly. This study shows that, in the hatching chick, the small intestine matures in a manner similar to neonatal mammals, with specific ontogenetic timetables in the different small intestinal segments, however, the most dramatic changes occur within the first 24 h posthatch.