Physical and physiological effects of stratum corneum tape stripping
Physical and physiological effects of stratum corneum tape stripping
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DOI：
10.1034/j.1600-0846.2001.007001040.x
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发表时间：
2001-02-01
影响因子：
2.2
通讯作者：
Maibach, HI
中科院分区：
文献类型：
--
作者：
Bashir, SJ;Chew, AL;Maibach, HI
背景/目标：已对人体角质层进行胶带剥离，以测量角质层质量、屏障功能、药物储存库和经皮渗透。然而，技术本身需要进一步发展，以方便interpretation.Methods：在这项研究中，我们量化角质层（SC）磁带剥离和水动力学参数，利用三种类型的胶带，在体内随机临床试验。用胶带剥离角质层，并利用蛋白质测定定量由每个胶带去除的SC的质量。测量经表皮水分损失（TEWL），并计算屏障破坏和SC水动力学。三种常用的丙烯酸酯胶带，利用和比较theme.Results：每种类型的磁带成功剥离角质层，但人造丝胶带没有引起SC屏障破坏。无论是磁带的类型，也没有网站剥离显着影响的SC删除的质量。水动力学参数没有显着不同的磁带，诱导屏障破坏。在屏障破坏水以下磁带stripping.Conclusion：磁带利用去除类似量的SC. The磁带有不同的倾向，导致屏障破坏的个体差异。一些人没有表现出增加的TEWL，尽管与那些表现出反应的人相比，去除了等量的SC。
Background/aims: Tape stripping of human stratum corneum has been performed to measure stratum corneum mass, barrier function, drug reservoir and percutaneous penetration. However, the technique itself requires further development to facilitate interpretation.Methods: In this study we quantified stratum corneum (SC) tape stripping and water kinetic parameters utilizing three types of adhesive tapes, in an in vivo randomized clinical trial. Stratum corneum was tape stripped, and the mass of SC removed by each tape was quantified utilizing a protein assay. Transepidermal water loss (TEWL) was measured and barrier disruption and SC water kinetics calculated. Three commonly utilized acrylate adhesive tapes were utilized and a comparison made between them.Results: Each type of tape successfully stripped the stratum corneum, but the rayon tape did not induce SC barrier disruption. Neither the type of tape nor the site stripped significantly influenced the mass of SC removed. Water kinetic parameters did not differ significantly for the tapes that did induce barrier disruption. Individual variation in barrier disruption to water following tape stripping was demonstrated.Conclusion: The tapes utilized removed a similar amount of SC. The tapes have a different propensity to cause barrier disruption. Some individuals do not demonstrate increased TEWL despite an equivalent mass of SC being removed compared to those who do show a response.