PbPDCB16-mediated callose deposition affects the plasmodesmata blockage and reduces lignification in pear fruit.
PbPDCB16-mediated callose deposition affects the plasmodesmata blockage and reduces lignification in pear fruit.
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DOI：
10.1016/j.plantsci.2023.111876
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Wen Li;Kaili Yuan;Mei Ren;Z. Xie;K. Qi;Xin Gong;Qi Wang;Shaoling Zhang;S. Tao
中科院分区：
文献类型：
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作者：
Wen Li;Kaili Yuan;Mei Ren;Z. Xie;K. Qi;Xin Gong;Qi Wang;Shaoling Zhang;S. Tao
石细胞是梨木化细胞的一种，是梨所特有的性状，是影响梨果实品质和经济价值的关键因素之一。细胞木质化过程的传递性已被证明存在，但胼胝质对胞间连丝(PD)通透性的影响以及如何影响细胞木质化过程仍不清楚。本研究对梨基因组中PD胼胝质结合蛋白（PDCB）基因家族进行了全基因组分析，鉴定出25个PbPDCB基因，并将其分为4个分支。在同一分支中观察到类似的内含子/外显子结构模式，有力地支持了它们密切的进化关系。 qRT-PCR分析发现PbPDCB16的表达与木质素积累呈负相关。在梨果实中瞬时表达，在梨愈伤组织中稳定表达，进一步观察到胼胝质含量增加，木质素含量下降。此外，与野生型拟南芥相比，转基因植株生长缓慢，茎部细胞壁更薄，细胞壁上的PD较少，通过透射电镜（TEM）观察，转基因拟南芥中的孢子间丝也被阻断。综上所述，PbPDCB16的过表达可以促进PD处胼胝质的积累，影响PD介导的细胞间连接，抑制细胞间通讯。该研究将为通过胼胝质沉积降低木质素含量提供新的思路，也为进一步探索梨果实木质素代谢和细胞壁木质化形成石细胞提供理论基础。
Stone cell, a type of lignified cell, is a unique trait in pear and one of the key factors affects pear fruit quality and economic value. The transmissibility of cell lignification process has been proven to exist, however the effects of callose on the permeability of plasmodesmata (PD) and how to influence cell lignification processes are still unknown. In this study, the genome-wide analysis of PD callose binding proteins (PDCB) gene family in pear genome was performed, and 25PbPDCBgenes were identified and divided into four branches. Similar intron/exon structural patterns were observed in the same branch, strongly supporting their close evolutionary relationship. The expression ofPbPDCB16was negatively correlated with lignin accumulation through qRT-PCR analysis.With transient expression in pear fruit and stable expression in pearcalli, the increased callose content accompanied by decreased lignin content was further observed. Besides, compared with wild type Arabidopsis, the transgenic plants grew slowly, and cell walls in the stem were thinner, while fewer PDs were observed on the cell walls, and the interspore filaments were also blocked in transgenicArabidopsisthrough the transmission electron microscope (TEM). In summary, overexpression ofPbPDCB16could promote accumulation of callose at PD to affect the PD-mediated intercellular connectivity, and inhibit the intercellular communication. This study will provide new insight in reducing the lignin content through callose deposition, and also provide the theoretical basis for further exploration of lignin metabolism and cell wall lignification to form stone cells in pear fruit.