In Situ Biosensing with a Surface Plasmon Resonance Fiber Grating Aptasensor
In Situ Biosensing with a Surface Plasmon Resonance Fiber Grating Aptasensor
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DOI：
10.1021/ac201641n
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发表时间：
2011-09-15
影响因子：
7.4
通讯作者：
Albert, Jacques
中科院分区：
文献类型：
--
作者：
Shevchenko, Yanina;Francis, Tariq J.;Albert, Jacques
使用光纤制备的表面等离子体共振（SPR）生物传感器可以用作用于原位或可能在体内监测生物分子相互作用的良好建立的生物传感器平台的具有成本效益且相对易于实施的替代方案。在这项研究中提出的光纤生物传感器利用一个在光纤倾斜布拉格光栅激发表面上的SPR的传感器在很大范围的外部介质的折射率，最小的交叉灵敏度的温度和不损害光纤的结构完整性。所使用的无标记生物识别方案表明，传感器依赖于用适体对镀金纤维进行功能化，适体是以高特异性结合给定靶标的合成DNA序列。除了通过适体实时监测纤维的功能化之外，结果还显示了纤维生物传感器如何在缓冲液和血清溶液中的各种浓度的凝血酶中检测适体靶标的存在。研究结果还显示了SPR生物传感器如何用于评估解离常数（Kd），因为结合常数与文献中已经报道的值一致。
Surface plasmon resonance (SPR) biosensors prepared using optical fibers can be used as a cost-effective and relatively simple-to-implement alternative to well established biosensor platforms for monitoring biomolecular interactions in situ or possibly in vivo. The fiber biosensor presented in this study utilizes an in-fiber tilted Bragg grating to excite the SPR on the surface of the sensor over a large range of external medium refractive indices, with minimal cross-sensitivity to temperature and without compromising the structural integrity of the fiber. The label-free biorecognition scheme used demonstrates that the sensor relies on the functionalization of the gold-coated fiber with aptamers, synthetic DNA sequences that bind with high specificity to a given target. In addition to monitoring the functionalization of the fiber by the aptarners in real-time, the results also show how the fiber biosensor can detect the presence of the aptamer's target, in various concentrations of thrombin in buffer and serum solutions. The findings also show how the SPR biosensor can be used to evaluate the dissociation constant (K-d), as the binding constant agrees with values already reported in the literature.