Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit
Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit
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DOI：
10.5511/plantbiotechnology.19.345
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发表时间：
2002-12
影响因子：
1.6
通讯作者：
H. Kunitake;Kohji Nagasawa;K. Takami;H. Komatsu
中科院分区：
文献类型：
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作者：
H. Kunitake;Kohji Nagasawa;K. Takami;H. Komatsu
将将军柑（Citrus reticulata布兰科，2n = 2x = 18）胚性愈伤组织原生质体与葡萄柚（C. paradisi Macf.，2n = 2x =18）。利用RAPD和PCR-RFLP技术对再生植株的叶片性状和核基因组进行分析，证实了两个再生植株的杂种性。流式细胞仪和根尖细胞计数的两个杂种揭示了染色体数目为27。这些三倍体体细胞杂种转入田间后，形态正常，生长旺盛。田间表现和抗病性正在调查中。
Somatic hybrids were produced between embryogenic callus protoplasts of ‘Shogun’ mandarin (Citrus reticulata Blanco, 2n = 2x = 18) and leaf protoplasts of grapefruit (C. paradisi Macf., 2n = 2x =18) by electrofusion. Hybridity of the two regenerated plants was confirmed by leaf characteristics and nuclear genome analysis using RAPD and PCR-RFLP. Flow cytometry and root-tip cell count of the two hybrids revealed a chromosome number of 27. These triploid somatic hybrids on transfer to field showed normal morphology and vigorous growth. Field performances as well as disease resistance are under investigation.