Comparison of partial citrate synthase gene (gltA) sequences for phylogenetic analysis of Bartonella species
Comparison of partial citrate synthase gene (gltA) sequences for phylogenetic analysis of Bartonella species
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DOI：
10.1099/00207713-46-4-891
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发表时间：
1996-10-01
期刊：
影响因子：
--
通讯作者：
Raoult, D
中科院分区：
文献类型：
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作者：
Birtles, RJ;Raoult, D
获得了八种有效描述的巴尔通体属物种和七种从小型哺乳动物获得的未表征的巴尔通体属菌株的代表性的柠檬酸脱氢酶编码基因（gltA）的940-bp片段的核苷酸碱基序列数据。还确定了未表征的菌株的完整16 S rRNA基因序列，并且这些序列显示每个菌株具有与先前识别的Bautonella物种的序列非常相似的独特序列，不同巴尔通体属物种的gltA序列的比较显示，序列之间的相似性水平为83.8%至93.5%，而同一种不同菌株间的序列比较表明，其相似性均在99.8%以上。其中1株的glfA基因序列与伊丽莎白巴尔通体的glfA基因序列一致，3株的glfA基因序列相似性大于99.6%（但与任何其他序列的相似性小于93.5%），其余三个：未表征的菌株各自表现出与其它巴尔通体属物种或分离株小于93.5%的序列相似性，系统发育树推断gltA和16 S核糖体DNA（rDNA）序列的多重比对。然而，通过使用距离矩阵和简约方法从16 S rDNA序列数据推断的树的所提出的巴尔通体内结构实际上没有统计学支持，从gltA序列数据推断的树包含该属中的四个支持良好的谱系，gltA-在研究植物的进化关系时，165 rDNA序列数据的同源性似乎比165 rDNA序列数据的同源性更有用。巴尔通体种，和gltA分析所确定的谱系的有效性进行了讨论。
Nucleotide base sequence data were obtained for a 940-bp fragment of the citrate synthase-encoding gene (gltA) of representatives of the eight validly described Bartonella species and seven uncharacterized Bartonella strains obtained from small mammals, Complete 16S rRNA gene sequences were also determined for the uncharacterized strains, and these sequences revealed that each strain had a unique sequence which was very similar to the sequences of the previously recognized Bautonella species, A comparison of the gltA sequences of the different Bartonella species revealed that the levels of similarity between sequences were 83.8 to 93.5%, whereas comparisons of sequences obtained from different strains of the same species revealed that the levels of similarity were more than 99.8%. One of the uncharacterized strains had a glfA sequence that matched the sequence of Bartonella elizabethae, three uncharacterized strains had sequences which were more than 99.6% similar to each other (but less than 93.5% similar to any other sequence), and the remaining three: uncharacterized strains each exhibited less than 93.5% sequence similarity to other Bartonella species or isolates, Phylogenetic trees were inferred from multiple alignments of both gltA and 16S ribosomal DNA (rDNA) sequences. Whereas the proposed intra-Bartonella architecture of trees inferred from 16S rDNA sequence data by using both distance matrix and parsimony methods had virtually no statistical support, the trees inferred from the gltA sequence data contained four well-supported lineages in the genus, The gltA-derived phylogeny appears to be more useful than the phylogeny derived from 165 rDNA sequence data for investigating the evolutionary relationships of Bartonella species, and the validity of the lineages identified by the gltA analysis is discussed in this paper.