Establishment of a cell line from the platyfish-swordtail hybrid melanoma.
Establishment of a cell line from the platyfish-swordtail hybrid melanoma.
复制标题
建立来自鸭嘴鱼-剑尾杂交黑色素瘤的细胞系。
作者：
Y. Wakamatsu
从剑尾鱼种间杂种的遗传性黑色素瘤中建立了一个鱼黑色素瘤细胞系PSM-1，用于该黑色素瘤系统的细胞遗传学研究。一个无黑色素性黑色素瘤是从一个黑色素的F1杂交斑点扁鱼（剑尾鱼maculatus）和一个白化病剑尾鱼（剑尾鱼helleri）。将肿瘤组织分离并在补充有10%胎牛血清的Eagle最小必需培养基中培养。自1978年3月3日起，PSM-1已在体外培养了52代和29个月。该细胞系在细胞形态上显示出相当大的变化。光镜下未见明显色素，电镜下可见黑素体和前黑素体。细胞彼此随机生长，明显缺乏接触抑制，并形成许多团块。加倍时间约为2天，第41次传代时的众数接近三体71。检测到高酪氨酸酶活性。PSM-1在细胞学特征和生长模式方面与一些哺乳动物黑色素瘤细胞系相似。
A fish melanoma cell line, PSM-1, was established from a hereditary melanoma in an interspecific hybrid of Xiphophorus for cytogenetic studies of this melanoma system. An amelanotic melanoma was obtained from the tail fin of a melanotic F1 hybrid between a spotted platyfish (Xiphophorus maculatus) and an albino swordtail (Xiphophorus helleri). The tumor tissue was dissociated and cultured in Eagle's minimal essential medium supplemented with 10% fetal calf serum. PSM-1 has been maintained for 52 passages and 29 months in vitro since March 3, 1978. This cell line showed considerable variation in cellular morphology. Although no apparent pigment was detectable by light microscopy, melanosomes and premelanosomes could be seen under the electron microscope. The cells grew randomly across each other with an apparent lack of contact inhibition and formed many clumps. The doubling time was approximately 2 days, and the modal chromosome number at the 41st passage was a near triploid 71. A high tyrosinase activity was detected. PSM-1 is similar to some mammalian melanoma cell lines with respect to cytological characteristics and growth patterns.