Screening of functionalized collagen membranes with a porcine periodontal regeneration model
Screening of functionalized collagen membranes with a porcine periodontal regeneration model
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DOI：
10.1111/odi.14445
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发表时间：
2022-12-02
期刊：
影响因子：
3.8
通讯作者：
Sone，Eli D.
中科院分区：
文献类型：
--
作者：
Quan，Bryan D.;Sadeghi，Rokhsareh;Sone，Eli D.
目的：目前的牙周再生方法不能促进胶原纤维嵌入新骨和牙骨质。我们使用猪伤口模型来筛选不同的功能化胶原蛋白膜，以促进牙周重新附着到根surfaces.MethodsTreatment组包括（1）对照无膜，（2）胶原蛋白涂层膜，（3）膜与胰岛素样生长因子-1（IGF-1），（4）膜与amelotin，或（5）膜附着磷酸钙骨水泥（CPC），或与CPC结合IGF-1。皮瓣程序进行下颌和上颌前磨牙的每一个pig.ResultsHistomorphometric，micro-CT，并在4和12周后获得的临床测量结果显示，牙骨质形成裸露的根和牙槽骨的重建，表明猪模型可以在牙周再生愈合反应模型。与其他治疗方法相比，磷酸钙骨水泥由于无需缝合而简化了手术，并改善了牙槽骨的再生（p< 0.05）。仅IGF组的PD降低（p< 0.05）。治疗组之间观察到的大的差异表明，先验功率分析应进行优化statistical analysis.ConclusionsPigs可以使用基于胶原蛋白的功能化膜牙周愈合的离散元素建模。筛选结果表明，磷酸钙骨水泥膜支抗促进牙槽骨再生。
ObjectivesCurrent methods for periodontal regeneration do not promote collagen fiber insertions into new bone and cementum. We used a pig wound model to screen different functionalized collagen membranes in promoting periodontal reattachment to root surfaces.MethodsTreatment groups included (1) control with no membranes, (2) collagen‐coated membranes, (3) membranes with insulin‐like growth factor‐1 (IGF‐1), (4) membranes with amelotin, or (5) membranes attached with calcium phosphate cement (CPC), or with CPC combined with IGF‐1. Flap procedures were performed on mandibular and maxillary premolars of each pig.ResultsHistomorphometric, micro‐CT, and clinical measurements obtained at 4 and 12 weeks after surgery showed cementum formation on denuded roots and reformation of alveolar bone, indicating that the pig model can model healing responses in periodontal regeneration. Calcium phosphate cement simplified procedures by eliminating the need for sutures and improved regeneration of alveolar bone (p< 0.05) compared with other treatments. There was a reduction (p< 0.05) of PD only for the IGF group. Large observed variances between treatment groups indicated that a priori power analyses should be conducted to optimize statistical analysis.ConclusionsPigs can model discrete elements of periodontal healing using collagen‐based, functionalized membranes. Screening indicates that membrane anchorage with calcium phosphate cements improve regeneration of alveolar bone.