Photosensitizer encapsulated organically modified silica nanoparticles for direct two-photon photodynamic therapy and In Vivo functional imaging
Photosensitizer encapsulated organically modified silica nanoparticles for direct two-photon photodynamic therapy and In Vivo functional imaging
复制标题
用于直接双光子光动力治疗和体内功能成像的光敏剂封装有机改性二氧化硅纳米颗粒
DOI：
10.1016/j.biomaterials.2012.02.053
复制
发表时间：
2012-06-01
期刊：
影响因子：
14
通讯作者：
He, Sailing
中科院分区：
文献类型：
--
作者：
Qian, Jun;Wang, Dan;He, Sailing
纳米颗粒辅助双光子成像和近红外（NIR）成像是生物光子学研究中的两项重要技术。在本文中，合成了用 PpIX（原卟啉 IX）光敏剂或 IR-820 NIR 荧光团封装的有机改性二氧化硅 (ORMOSIL) 纳米颗粒，并进行了光学表征。使用以前的 ORMOSIL 纳米颗粒，我们展示了：（i）通过其在肿瘤细胞内环境中有效的双光子吸收直接激发 PpIX 的荧光，以及（ii）PpIX 在双光子照射下对肿瘤细胞的细胞毒性。后者的 ORMOSIL 纳米粒子可用作有效的近红外荧光造影剂，用于各种类型的体内动物成像。我们将 IR-820 掺杂的 ORMOSIL 纳米颗粒应用于小鼠体内脑成像。我们还展示了它们在小鼠前哨淋巴结（SLN）绘图中的应用。最后，我们证明纳米探针可以针对小鼠皮下异种移植肿瘤进行长时间观察。 ORMOSIL 纳米粒子在疾病诊断和临床治疗方面具有巨大的潜力。 (c) 2012 Elsevier Ltd. 保留所有权利。
Nanoparticle-assisted two-photon imaging and near infrared (NIR) imaging are two important technologies in biophotonics research. In the present paper, organically modified silica (ORMOSIL) nanoparticles encapsulated with either PpIX (protoporphyrin IX) photosensitizers or IR-820 NIR fluorophores were synthesized and optically characterized. Using the former ORMOSIL nanoparticles, we showed: (i) direct excitation of the fluorescence of PpIX through its efficient two-photon absorption in the intracellular environment of tumor cells, and (ii) cytotoxicity towards tumor cells by PpIX under two-photon irradiation. The latter ORMOSIL nanoparticles can be used as efficient NIR fluorescent contrast agents for various types in vivo animal imaging. We applied IR-820 doped ORMOSIL nanoparticles in in vivo brain imaging of mice. We also demonstrated the applications of them to sentinel lymph node (SLN) mapping of mice. Finally, we showed that the nanoprobes could target the subcutaneously xenografted tumor of a mouse for long time observations. ORMOSIL nanoparticles have great potentials for disease diagnosis and clinical therapies. (c) 2012 Elsevier Ltd. All rights reserved.