Overexpression of Arabidopsis and Rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice
Overexpression of Arabidopsis and Rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice
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DOI：
10.1111/j.1467-7652.2012.00688.x
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发表时间：
2012-06-01
影响因子：
13.8
通讯作者：
Datta, Swapan K.
中科院分区：
文献类型：
--
作者：
Datta, Karabi;Baisakh, Niranjan;Datta, Swapan K.
水稻产量受干旱、盐碱等环境胁迫的影响很大。为了应对水稻耐这些胁迫的挑战，我们引入了在胁迫诱导型rd29启动子控制下编码转录因子DREB1A和DREB1B的cDNA。使用了两种不同的籼稻品种，BR29是来自孟加拉国的改良商业栽培品种，IR68899B是国际水稻研究所培育的杂交水稻保持系。农杆菌介导的BR29转化是用从水稻和拟南芥中分离的DREB1A和从水稻中分离的DREB1B进行的，而在IR68899B的情况下，用水稻- DREB1B进行了生物转化。PCR和Southern blot分析证实了初步的遗传整合。对幼苗进行耐盐性试验。在开花前孕穗期进行干旱胁迫试验更为可靠。采用RNA凝胶印迹分析和定量PCR分析来估计胁迫和非胁迫条件下的转录水平。对胁迫和未胁迫植株进行了农艺性能研究，比较了脱水和盐负荷胁迫造成的产量损失。从拟南芥中分离的DREB1A转化的植株对脱水的耐受性明显增强，而DREB1B的耐盐性更强。
Rice yield is greatly affected by environmental stresses such as drought and salinity. In response to the challenge of producing rice plants tolerant to these stresses, we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter. Two different indica rice cultivars were used, BR29, an improved commercially cultivated variety from Bangladesh and IR68899B, an IRRI bred maintainer line for hybrid rice. Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice, whereas biolistic transformation was done with rice- DREB1B in the case of IR68899B. Initial genetic integration was confirmed by PCR and Southern blot analysis. Salinity tolerance was assayed in very young seedlings. Drought stress tests were found to be more reliable when they were carried out at the pre-flowering booting stage. RNA gel blot analysis as well as quantitative PCR analysis was performed to estimate the transcription level under stressed and unstressed conditions. Agronomic performance studies were done with stressed and unstressed plants to compare the yield losses due to dehydration and salt loading stresses. Noticeably enhanced tolerance to dehydration was observed in the plants transformed with DREB1A isolated from Arabidopsis while DREB1B was found to be more effective for salt tolerance.