Determination of clenbuterol from pork samples using surface molecularly imprinted polymers as the selective sorbents for microextraction in packed syringe
Determination of clenbuterol from pork samples using surface molecularly imprinted polymers as the selective sorbents for microextraction in packed syringe
复制标题
使用表面分子印迹聚合物作为包装注射器中微萃取的选择性吸附剂测定猪肉样品中的克伦特罗
DOI：
10.1016/j.jpba.2013.12.022
复制
发表时间：
2014-03-25
影响因子：
3.4
通讯作者：
Chang, Chun
中科院分区：
文献类型：
--
作者：
Du, Wei;Lei, Chunmei;Chang, Chun
建立了一种表面分子印迹聚合物与填充式注射器微萃取（SMIPs-MEPS）相结合的选择性样品前处理方法，用于猪肉样品中克伦特罗（CLB）的分析。采用溶胶-凝胶法在硅胶颗粒上合成了用于CLB的SMIPs。一系列表征和吸附实验表明，SMIPs具有多孔结构，良好的热稳定性，高吸附容量和快速的传质速率。将所制备的SMIPs作为SMIPs-MEPS的选择性吸附剂用于猪肉样品中CLB的提取。研究了影响萃取效率的几个参数，包括样品溶液的pH值、抽-排循环次数、样品体积、洗涤溶液的类型和体积以及洗脱溶液的类型和体积。在优化的条件下，建立了猪肉中CLB的高效液相色谱分析方法。整个预处理过程是快速的，它可以在2分钟内完成。CLB的定量限和检测限分别为0.02和0.009 μ g kg（-1），分别。3个添加水平的平均回收率在86.5%~ 91.2%之间，相对标准偏差（RSD）为0.99%。
In this study, a selective sample pretreatment procedure combing surface molecularly imprinted polymers and microextraction in packed syringe (SMIPs-MEPS) was developed for the analysis of clenbuterol (CLB) from pork samples. SMIPs for CLB were synthesized on silica gel particles through a sal-gel process. A series of characterization and adsorption experiments revealed that the SMIPs exhibited porous structures, good thermal stability, high adsorption capacity and a fast mass transfer rate. The obtained SMIPs were employed as selective sorbents of SMIPs-MEPS for extraction of CLB from pork samples. Several parameters affecting the extraction efficiency were investigated, including the pH of sample solution, number of draw-eject cycles, volume of sample, type and volume of washing solution, and the type and volume of elution solution. Under the optimized conditions, a simple and rapid method for the determination of CLB from pork samples was established by coupling with high performance liquid chromatography (HPLC). The whole pretreatment process was rapid and it can be accomplished with 2 min. The limit of quantitation and the limit of detection for CLB were 0.02 and 0.009 mu g kg(-1), respectively. The average recoveries of CLB at three spiked levels ranged from 86.5% to 91.2% with the relative standard deviations (RSD)