Resazurin assay of radiation response in cultured cells
Resazurin assay of radiation response in cultured cells
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DOI：
10.1259/bjr/54004230
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发表时间：
2005-10-01
影响因子：
2.6
通讯作者：
Allshire, A
中科院分区：
文献类型：
--
作者：
Anoopkumar-Dukie, S;Carey, JB;Allshire, A
我们描述了使用瑞祖啉还原来测量细胞对辐射的反应，作为一种简单和非破坏性的测定，补充了传统的集落形成测定，可以很容易地应用于贴壁和非贴壁细胞培养。reazurin方法产生的数据与菌落形成试验以及DNA合成试验（BrdU结合）相当，在60%等效水平下，OER（氧增强比）为2.5，而菌落形成试验为3.1。瑞祖脲检测的日内和日间精密度分别为4.1%和5.2%。
We describe use of resazurin reduction for measurement of cell response to irradiation as a simple and non-destructive assay that complements the conventional colony forming assay and can readily be applied to both adherent and non-adherent cell cultures. The resazurin method yields data comparable with the colony forming assay as well as to assay of DNA synthesis (BrdU incorporation), giving an OER (oxygen enhancement ratio) of 2.5 at 60% isoeffect level versus 3.1 for the colony forming assay. Intraday and interday precisions for the resazurin assay were 4.1% and 5.2%, respectively.