Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas
Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas
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DOI：
10.1080/09168451.2018.1448255
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发表时间：
2018-01-01
影响因子：
1.6
通讯作者：
Sambongi, Yoshihiro
中科院分区：
文献类型：
--
作者：
Fujii, Sotaro;Masanari-Fujii, Misa;Sambongi, Yoshihiro
两种细胞色素c(5) （SBcytc和SVcytc）来源于生活在深海高压环境中的希瓦氏菌，因此这两种蛋白质在高压下可能比在0.1MPa大气压下更稳定。然而，本研究发现，在0.1MPa下，SBcytc和SVcytc比在更高压力下更稳定。此外，在0.1 ~ 150mpa下，SBcytc和SVcytc的稳定性高于常压希瓦氏菌的同源物，这是由于前两种蛋白与血红素形成了氢键。本研究进一步揭示了深海假单胞菌的细胞色素c(551) （PMcytc）比常压铜绿假单胞菌的同系物更稳定，并与血红素形成特异性氢键。虽然SBcytc、SVcytc和PMcytc在系统发育上距离很远，但这些深海细胞色素c通常通过氢键形成来稳定。
Two cytochromes c(5) (SBcytc and SVcytc) have been derived from Shewanella living in the deep-sea, which is a high pressure environment, so it could be that these proteins are more stable at high pressure than at atmospheric pressure, 0.1MPa. This study, however, revealed that SBcytc and SVcytc were more stable at 0.1MPa than at higher pressure. In addition, at 0.1-150MPa, the stability of SBcytc and SVcytc was higher than that of homologues from atmospheric-pressure Shewanella, which was due to hydrogen bond formation with the heme in the former two proteins. This study further revealed that cytochrome c(551) (PMcytc) of deep-sea Pseudomonas was more stable than a homologue of atmospheric-pressure Pseudomonas aeruginosa, and that specific hydrogen bond formation with the heme also occurred in the former. Although SBcytc and SVcytc, and PMcytc are phylogenetically very distant, these deep-sea cytochromes c are commonly stabilized through hydrogen bond formation.