THE ASSOCIATION BETWEEN MI-2 ANTIBODIES AND DERMATOMYOSITIS
THE ASSOCIATION BETWEEN MI-2 ANTIBODIES AND DERMATOMYOSITIS
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DOI：
10.1002/art.1780280711
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发表时间：
1985-01-01
影响因子：
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通讯作者：
REICHLIN, M
中科院分区：
文献类型：
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作者：
TARGOFF, IN;REICHLIN, M
小牛胸腺提取物中的抗原Mi抗体在多发性肌炎（PM）和皮肌炎（DM）血清中被证明具有补体固定抑制作用，但在没有肌炎的个体血清中没有。最初的Mi参考血清定义了2种沉淀抗体，使用免疫扩散（ID）。抗m -1在补体固定中不活跃。进一步研究了Mi-2抗体，该抗体可能是Mi血清中修复补体的抗体。采用免疫亲和层析纯化Mi-2抗原。采用酶联免疫吸附试验（ELISA）测定Mi-2抗体，对139例肌炎患者血清进行检测，其中DM 52例，PM 87例。同时对35名正常人和93名其他结缔组织疾病患者的对照血清进行了检测。只有13份血清被认为是抗mi -2阳性。所有患者均为肌炎患者，其中11例为糖尿病患者。只有糖尿病患者血清中有抗mi -2抗体，ELISA检测结果均为阳性。许多其他血清，包括许多来自其他结缔组织疾病患者的血清和2个来自正常受试者的血清（均无沉淀抗体）的血清水平较低，其意义不确定。抗- mi -2在糖尿病患者中的检测频率明显高于PM患者。抗mi -2似乎与糖尿病密切相关，并且是这种形式的肌炎的第一个特异性血清学标记物。
Antibodies to Mi, an antigen in calf thymus extract, were demonstrated by complement fixation inhibition in polymyositis (PM) and dermatomyositis (DM) sera but not in the sera of individuals without myositis. The original Mi reference serum defined 2 precipitating antibodies, using immunodiffusion (ID). Anti-M-1 was not active in complement fixation. Anti-Mi-2, which appears to be the antibody in Mi serum that fixes complement was studied in further detail. Mi-2 antigen was purified by immunoaffinity chromatography. An enzyme-linked immunosorbent assay (ELISA) to measure Mi-2 antibody, using this antigen, was used to test the sera of 139 myositis patients: 52 had DM and 87 had PM. Control sera from 35 normal subjects and 93 patients with other connective tissue diseases were also tested. Only 13 sera were considered definitely positive for anti-Mi-2. All were from patients who had myositis, 11 of whom had DM. Only DM sera had anti-Mi-2 by ID, and all sera with anti-Mi-2 by ID were positive by ELISA. A number of other sera, including many from patients with other connective tissue diseases and 2 from normal subjects (all without precipitating antibodies) had lower elevations which were of uncertain significance. Detection of anti-Mi-2 by ID as well as by ELISA was significantly more frequent in DM than in PM. Anti-Mi-2 appears to be closely linked to DM, and is the first specific serologic marker for this form of myositis.