A murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex
A murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex
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一种识别人巨细胞病毒病毒粒子包膜糖蛋白复合物内单个糖蛋白的鼠单克隆抗体
DOI：
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发表时间：
1985
影响因子：
12.7
通讯作者：
G. Farrar
中科院分区：
文献类型：
--
作者：
K. Law;P. Wilton;G. Farrar
从高度纯化的人巨细胞病毒病毒粒子的非离子去污剂溶解的多肽被用作免疫原，以产生分泌鼠单克隆抗体的杂交瘤。通过免疫沉淀和随后的SDS-聚丙烯酰胺凝胶电泳（SDSPAGE）显示，一种单克隆抗体可沉淀分子量为52、95和130（均为× 103）的三种糖蛋白和一种分子量为50 × 103的次要组分。当首先通过SDS‐PAGE分离病毒体包膜组分并将其电泳转移到硝酸纤维素膜上时，该单克隆抗体识别分子量为50和52（均为× 103）的两种相关组分。免疫荧光研究表明，这些病毒抗原与病毒感染细胞的膜系统相关，并且在感染后期特别丰富。
Nonionic detergent solubilised polypeptides from highly purified human cytomegalovirus virions were used as immunogens to produce murine monoclonal antibody secreting hybridomas. One monoclonal antibody was shown, by immunoprecipitation followed by SDS‐polyacrylamide gel electrophoresis (SDSPAGE), to precipitate three glycoproteins with molecular weights 52, 95, and 130 (all × 103) and one minor component with a molecular weight of 50 × 103. When virion envelope components were first separated by SDS‐PAGE and electrophoretically transferred to nitrocellulose membranes, this monoclonal antibody recognised two related components with molecular weights 50 and 52 (both × 103). Immunofluorescence studies suggested that these viral antigens were associated with membrane systems of virus‐infected cells and were particularly abundant late in infection.
DOI：
10.1073/pnas.77.9.5307
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
THORLEYLAWSON, DA;GEILINGER, K
通讯作者：
GEILINGER, K