Evolution of bacterial community during bioremediation of PAHs in a coal tar contaminated soil
Evolution of bacterial community during bioremediation of PAHs in a coal tar contaminated soil
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DOI：
10.1016/j.chemosphere.2010.09.021
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发表时间：
2010-11-01
期刊：
影响因子：
8.8
通讯作者：
Damidot, Denis
中科院分区：
文献类型：
--
作者：
Lors, Christine;Ryngaert, Annemie;Damidot, Denis
通过跟踪多环芳烃浓度和细菌群落结构的演变，对煤焦油蒸馏产生的主要为2 - 3-和4-环多环芳烃污染土壤的料堆处理进行了监测。DGGE与细菌计数定量和16 PAH测量平行6个月的生物处理导致2- 3-和4-环PAH浓度大幅下降（分别为98 97和82%）这一结果与细菌PAH活性有关。降解菌主要属于γ-变形菌，特别是在处理过程中检测到的肠杆菌属和假单胞菌属。该组被认为是确定污染土壤中PAH潜在生物降解的良好生物指示剂。相反，在3天后检测到其他物种，如β-变形菌。2- 3-和4-环多环芳烃几乎完全降解的月份因此，β-变形菌群的存在可以被认为是一个很好的候选指标，以估计这类多环芳烃污染土壤的生物处理终点（C）2010 Elsevier Ltd版权所有
The monitoring of a windrow treatment applied to soil contaminated by mostly 2 3- and 4-ring PAHs produced by coal tar distillation was performed by following the evolution of both PAH concentration and the bacterial community Total and PAH-degrading bacterial community structures were followed by 165 rRNA PCR-DGGE in parallel with quantification by bacterial counts and 16 PAH measurements Six months of biological treatment led to a strong decrease in 2- 3- and 4-ring PAH concentrations (98 97 and 82% respectively) This result was associated with the activity of bacterial PAH-degraders belonging mainly to the Gamma proteobacteria in particular the Enterobacteria and Pseudomonas genera which were detected over the course of the treatment This group was considered to be a good bioindicator to determine the potential PAH biodegradation of contaminated soil Conversely other species like the Beta proteobacteria were detected after 3 months when 2- 3- and 4-ring PAHs were almost completely degraded Thus presence of the Beta proteobacteria group could be considered a good candidate indicator to estimate the endpoint of biotreatment of this type of PAH contaminated soil (C) 2010 Elsevier Ltd All rights reserved