Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement
Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement
复制标题
用于进行性运动的驱动蛋白 3 的颈部连接器的结构描绘。
DOI：
10.1016/j.jmb.2018.05.010
复制
发表时间：
2018-07-06
影响因子：
5.6
通讯作者：
Feng, Wei
中科院分区：
文献类型：
--
作者：
Ren, Jinqi;Zhang, Yong;Feng, Wei
进行性驱动蛋白马达含有一个颈连接器（NL），它介导化学机械耦合并控制方向性和持续性。然而，驱动蛋白-3 NL仍然很难确定，由于缺乏与以下颈部线圈（NC）的连接的结构信息。在这里，我们确定了KIF 13 B的马达结构域（MD）-NL-NCNT串联的结构，其定义了NL和NC之间的连接并描绘了驱动蛋白-3 NL。出乎意料的是，驱动蛋白-3NL的长度比先前预测的短得多。在MD-NL-NCNT结构中，NL以常规模式对接到MD上，但由于MD的较短N-末端覆盖链，NL与MD之间的相互作用相对较弱。最佳的短NL及其与MD的弱相互作用将产生紧密的头间应变，并促进NL脱离，这可能有助于驱动蛋白3的快速和超进行运动。(C)2018爱思唯尔有限公司版权所有。
Processive kinesin motors contain a neck linker (NL) that mediates the chemo-mechanical coupling and controls the directionality and processivity. However, kinesin-3 NL remains poorly determined due to the lack of the structural information of the junction with the following neck coil (NC). Here, we determined the structure of the motor domain (MD)-NL-NCNT tandem of KIF13B that defines the junction between NL and NC and delineates kinesin-3 NL. Unexpectedly, the length of kinesin-3 NL is much shorter than the previously predicted one. In the MD-NL-NCNT structure, NL docks onto the MD with a conventional mode but the interaction between NL and the MD is relatively weak due to the shorter N-terminal cover strand of the MD. The optimal short NL and its weak interaction with the MD would generate the tight inter-head strain and facilitate the NL undocking, which may contribute to the fast and superprocessive motility of kinesin-3. (C) 2018 Elsevier Ltd. All rights reserved.