The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens.
The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens.
复制标题
rsmS (ybaM) 突变导致肠细菌植物病原体中 RsmAB 转录后毒力调节系统的旁路抑制。
DOI：
10.1038/s41598-019-40970-3
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发表时间：
2019
影响因子：
4.6
通讯作者：
Monson RE
中科院分区：
文献类型：
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作者：
Monson RE
植物细胞壁降解酶（PCWDEs）是马铃薯病原菌黑腐果胶杆菌（Pectobacterium atrosepticum）等软腐细菌的主要毒力决定因子。次级代谢物（Rsm）系统的调节控制了多氯WDE的生产，以应对不断变化的营养条件。本工作鉴定了一个新的anrsmB突变抑制基因-ECA 1172 orrsmS（rsmBsuppressor）。inrsmB缺陷突变体（编码一种小的调节RNA）显示群体感应分子（N-3-氧代己酰-高丝氨酸内酯; OHHL）和PCWDE的加工减少。而anrsmB、rsmSdouble突变体的OHHL和PCWDE产量部分恢复。SinglersmSmutaments，overproduced PCWDEs and OHHL relative to wild type P.在马铃薯中表现出强毒力。RsmS的过量生产也导致多氯WDEs和OHHL的增加。同源性搜索揭示了病原体如大肠杆菌（ybaM）、立枯迪克氏菌（Dickeyasolani）、肺炎克雷伯氏菌（Klebsiellapneumoniae）和福氏志贺菌（Shigellaflexneri）的同源性。胡萝卜腐果胶杆菌ATCC 39048的一个rsmS突变体表现出绕过rsmB依赖的PCWDE和OHHL产生的抑制。胡萝卜腐果胶杆菌ATCC 39048产生β-内酰胺抗生素1-碳青霉烯-2-烯-3-羧酸（一种碳青霉烯）。抗生素的产生在anrsmB突变体中被抑制，但在anrsmB、rsmSdouble突变体中部分恢复。这项工作突出了RsmS作为毒力和抗生素生物合成的保守多效性调节剂的重要性。
Plant cell wall degrading enzymes (PCWDEs) are the primary virulence determinants of soft rotting bacteria such as the potato pathogen,Pectobacterium atrosepticum. Theregulation ofsecondarymetabolite (Rsm) system controls production of PCWDEs in response to changing nutrient conditions. This work identified a new suppressor of anrsmBmutation –ECA1172orrsmS(rsmBsuppressor). Mutants defective inrsmB(encoding a small regulatory RNA), show reduced elaboration of the quorum sensing molecule (N-3-oxohexanoyl-homoserine lactone; OHHL) and PCWDEs. However, OHHL and PCWDE production were partially restored in anrsmB, rsmSdouble mutant. SinglersmSmutants, overproduced PCWDEs and OHHL relative to wild typeP. atrosepticumand exhibited hypervirulence in potato. RsmS overproduction also resulted in increased PCWDEs and OHHL. Homology searches revealedrsmSconservation across pathogens such asEscherichia coli (ybaM),Dickeya solani, Klebsiella pneumoniaeandShigella flexneri. AnrsmSmutant ofPectobacterium carotovorumATCC39048 showed bypass ofrsmB-dependent repression of PCWDEs and OHHL production.P. carotovorumATCC39048 produces the β-lactam antibiotic, 1-carbapen-2-em-3-carboxylic acid (a carbapenem). Production of the antibiotic was repressed in anrsmBmutant but partially restored in anrsmB, rsmSdouble mutant. This work highlights the importance of RsmS, as a conserved pleiotropic regulator of virulence and antibiotic biosynthesis.