Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney
Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney
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DOI：
10.1007/s00011-008-7227-2
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发表时间：
2009-03-01
影响因子：
6.7
通讯作者：
Chung, H. Y.
中科院分区：
文献类型：
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作者：
Jung, K. J.;Lee, E. K.;Chung, H. Y.
为了比较短期热量限制（CR）与已知长期热量限制（CR）对衰老大鼠肾脏的抗炎作用，探讨了10天热量限制（CR）对衰老大鼠肾脏的抗炎作用。两个不同的年龄组，6个月（幼龄）和24个月（高龄）。老龄组1亚组为对照，自由采食（AL），另1亚组饲喂CR，采食量为AL亚组采食量的40%，饲喂10 d （n = 5）。测定活性物质（RS）、脂质过氧化物和COX-2活性。用EMSA测定促炎转录因子NF-kB和AP-1的活性。Western blot检测NF-kB和AP-1上游信号级联以及促炎基因的表达。10天CR抑制衰老大鼠肾脏RS、脂质过氧化物和COX-2活性。CR还抑制NF-kB和AP-1的上游信号级联和DNA结合活性，以及硫氧还蛋白/Ref-1通路。CR阻断nf - kb和ap -1应答基因COX-2、iNOS、VCAM-1和ICAM-1的表达。我们首次报道，10天的CR可以减弱炎症过程的信号转导，这是由rs诱导的NF-kB和AP-1介导的。
To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney.Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5).Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot.10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1.We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.