Molecular features of odorants systematically influence slow temporal responses across clusters of coordinated antennal lobe units in the moth Manduca sexta
Molecular features of odorants systematically influence slow temporal responses across clusters of coordinated antennal lobe units in the moth Manduca sexta
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DOI：
10.1152/jn.01132.2003
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发表时间：
2004-07-01
影响因子：
2.5
通讯作者：
Smith, BH
中科院分区：
文献类型：
--
作者：
Daly, KC;Wright, GA;Smith, BH
许多物种的嗅觉辨别和刺激泛化的行为学研究表明，单分子气味物质的分子特征对气味辨别很重要。在这里，我们评估的功能，如碳链长度和官能团，在突触处理的第一级表示。我们记录触角叶合奏蛾天蛾重复100毫秒脉冲的单分子醇和酮的反应。大多数单位表现出显着的变化，穗率响应大多数气味持续时间的刺激。然后对所有气味剂的每个脉冲在780 ms内采样刺激周数据。因子分析被用来评估是否有组的单位与共同的反应模式。我们发现，因素识别和代表活动的集群的单位具有共同的时间响应特性。这些时间模式化的响应通常跨越780 ms，并且通常取决于碳链长度和官能团。此外，交叉相关分析经常表明，即使在自发活动的重大重合尖峰。然而，这种同步主要发生在同一个四极管上记录的单元之间。在最后的分析中，使用因子作为维度计算每对气味剂的气味响应之间的欧几里得距离。任何两种气味的反应之间的距离最大化，由类似的240毫秒。这个时间过程对应于整个记录人口的协调突发的简短序列。在此期间的距离也是分子特征系统差异的函数。因此，这种欧几里德分析的结果直接相关的刺激泛化在M。六。
Behavioral studies of olfactory discrimination and stimulus generalization in many species indicate that the molecular features of monomolecular odorants are important for odor discrimination. Here we evaluate how features, such as carbon chain length and functional group, are represented in the first level of synaptic processing. We recorded antennal lobe ensemble responses in the moth Manduca sexta to repeated 100-ms pulses of monomolecular alcohols and ketones. Most units exhibited a significant change in spike rate in response to most odorants that outlasted the duration of the stimulus. Peristimulus data were then sampled over 780 ms for each pulse of all odorants. Factor analysis was used to assess whether there were groups of units with common response patterns. We found that factors identified and represented activity for clusters of units with common temporal response characteristics. These temporally patterned responses typically spanned 780 ms and were often dependent on carbon chain length and functional group. Furthermore, cross-correlation analysis frequently indicated significant coincident spiking even during spontaneous activity. However, this synchrony occurred mainly between units recorded on the same tetrode. In a final analysis, the Euclidean distance between odor responses was calculated for each pair of odorants using factors as dimensions. The distance between responses for any two odorants was maximized by similar to 240 ms. This time course corresponded to the brief sequence of coordinated bursts across the recorded population. The distance during this period was also a function of systematic differences in molecular features. Results of this Euclidian analysis thus directly correlate to previous behavioral studies of stimulus generalization in M. sexta.