Linking Post-Translational Modifications and Variation of Phenotypic Traits
Linking Post-Translational Modifications and Variation of Phenotypic Traits
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DOI：
10.1074/mcp.m112.024349
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发表时间：
2013-03-01
影响因子：
7
通讯作者：
Sicard, Delphine
中科院分区：
文献类型：
--
作者：
Albertin, Warren;Marullo, Philippe;Sicard, Delphine
酶可以被翻译后修饰，导致具有不同性质的异构体。亚型的数量可变性的表型结果从未被研究过。我们用定量蛋白质组学的方法分析了酿酒酵母酒精发酵过程中酶的丰度与异构体、代谢性状和生长相关性状的关系。尽管分配给发酵蛋白质组的酶库在培养基和所考虑的菌株中是恒定的，但单个酶的丰度存在变化，有时其亚型的丰度要多得多，这表明存在对总蛋白丰度的选择性限制和亚型之间的权衡。某些异构体丰度的变化与代谢性状和生长相关性状显著相关。特别是，细胞大小和最大种群大小与乙醇脱氢酶的N-末端乙酰化程度高度相关。发酵蛋白质组被发现是由人类选择形成的，通过针对每个食品加工来源的菌株的几个异构体的差异化靶标。这些结果突出了翻译后修饰在代谢和生活史特征多样性中的重要性。分子与细胞蛋白质组学12：10.1074/mcp.M112.024349,720-735,2013年。
Enzymes can be post-translationally modified, leading to isoforms with different properties. The phenotypic consequences of the quantitative variability of isoforms have never been studied. We used quantitative proteomics to dissect the relationships between the abundances of the enzymes and isoforms of alcoholic fermentation, metabolic traits, and growth-related traits in Saccharomyces cerevisiae. Although the enzymatic pool allocated to the fermentation proteome was constant over the culture media and the strains considered, there was variation in abundance of individual enzymes and sometimes much more of their isoforms, which suggests the existence of selective constraints on total protein abundance and trade-offs between isoforms. Variations in abundance of some isoforms were significantly associated to metabolic traits and growth-related traits. In particular, cell size and maximum population size were highly correlated to the degree of N-terminal acetylation of the alcohol dehydrogenase. The fermentation proteome was found to be shaped by human selection, through the differential targeting of a few isoforms for each food-processing origin of strains. These results highlight the importance of post-translational modifications in the diversity of metabolic and life-history traits. Molecular & Cellular Proteomics 12: 10.1074/mcp.M112.024349, 720-735, 2013.