Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis
Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis
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DOI：
10.1016/j.mimet.2009.03.007
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Lan, Ruiting
中科院分区：
文献类型：
--
作者：
Chan, Wai-Fong;Maharjan, Ram P.;Lan, Ruiting
使用真实的时间PCR（RT-PCR）和高分辨率熔解（HRM）分析来快速分型编码百日咳无细胞疫苗组分的基因，即prn、ptxA、fhaB、fim 2和fim 3。采用RT-PCR结合HRM技术检测prn基因的长度多态性;采用发夹引物RT-PCR检测prn及其他基因的单核苷酸多态性。这些快速方法适用于疫苗驱动的百日咳杆菌进化的大规模研究。(C)2009爱思唯尔有限公司版权所有。
Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis. (C) 2009 Elsevier B.V. All rights reserved.