A standardized extract from Paeonia lactiflora and Astragalus membranaceus induces apoptosis and inhibits the proliferation, migration and invasion of human hepatoma cell lines
A standardized extract from Paeonia lactiflora and Astragalus membranaceus induces apoptosis and inhibits the proliferation, migration and invasion of human hepatoma cell lines
复制标题
芍药和黄芪标准化提取物诱导人肝癌细胞系凋亡并抑制增殖、迁移和侵袭
DOI：
10.3892/ijo.2013.2085
复制
发表时间：
2013-11-01
影响因子：
5.2
通讯作者：
Wei, Wei
中科院分区：
文献类型：
--
作者：
Wu, Jing-Jing;Sun, Wu-Yi;Wei, Wei
芍药和黄芪是两种中药，是治疗肝病的中药方中常用的药物。先前的研究已证明从芍药和黄芪（PAE）的根中提取的提取物对肝纤维化具有保护作用。然而，迄今为止尚未研究其对肝细胞癌（HCC）的影响。本研究探讨PAE对人肝癌细胞系HepG2和SMMC-7721凋亡、增殖、迁移和侵袭的影响。我们的数据表明，PAE (50-200 mg/l) 处理对肝癌细胞系 HepG2 和 SMMC-7721 的增殖产生抑制作用。此外，PI染色证实PAE诱导HepG2细胞和SMMC-7721细胞凋亡。此外，免疫细胞化学和蛋白质印迹显示，PAE处理后HepG2细胞和SMMC-7721细胞中Bcl-2的表达显着降低，而Bax和cleaved caspase-3的表达显着增加。这些结果清楚地表明PAE通过增加Bax与Bcl-2的比率并上调caspase-3的激活来诱导肝癌细胞凋亡。此外，伤口愈合实验和Matrigel侵袭实验的结果表明，PAE对HCC细胞的迁移和侵袭表现出抑制活性。综上所述，目前的数据证明 PAE 是治疗 HCC 的有效抗肿瘤候选药物。
Paeonia lactiflora and Astragalus membranaceus are two traditional Chinese medicines, which are commonly used in Chinese herb prescription to treat liver diseases. The protective effects of the extract prepared from the roots of Paeonia lactiflora and Astragalus membranaceus (PAE) on liver fibrosis have been demonstrated in previous studies. However, its effect on hepatocellular carcinoma (HCC) has not been investigated to date. In this study, the effects of PAE on the apoptosis, proliferation, migration and invasion of the human hepatoma cell lines HepG2 and SMMC-7721 were investigated. Our data demonstrated that treatment with PAE (50-200 mg/l) caused an inhibitory effect on the proliferation of the hepatoma cell lines HepG2 and SMMC-7721. Furthermore, PAE induced apoptosis of HepG2 cells and SMMC-7721 cells, which was demonstrated by PI staining. In addition, immunocytochemistry and western blotting showed that PAE significantly decreased the expression of Bcl-2, while the expression of Bax and cleaved caspase-3 in HepG2 cells and SMMC-7721 cells was significantly increased after treatment with PAE. These results clearly demonstrated that PAE induced hepatoma cell apoptosis through increasing the Bax-to-Bcl-2 ratio and upregulating the activation of caspase-3. In addition, the results of wound healing assay and Matrigel invasion assay showed that PAE displayed inhibitory activity on the migration and invasion of HCC cells. Taken together, the present data provides evidence that PAE is a potent antineoplastic drug candidate for the treatment of HCC.