Growth and magnetic resonance characteristics of human squamous cell carcinoma xenografts implanted with cells suspended in Matrigel.
Growth and magnetic resonance characteristics of human squamous cell carcinoma xenografts implanted with cells suspended in Matrigel.
复制标题
植入悬浮在基质胶中的细胞的人鳞状细胞癌异种移植物的生长和磁共振特征。
DOI：
10.1002/nbm.1940060503
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发表时间：
1993
影响因子：
2.9
通讯作者：
Weichselbaum,R
中科院分区：
文献类型：
--
作者：
Czerski,L;Majors,A;Ng,TC;Vijayakumar,S;Weichselbaum,R
本文研究了人鳞状细胞癌SQ 20 B在nu/nu裸鼠体内移植瘤的生长和磁共振特征。比较使用MatrigelTM（MTG，基底膜蛋白的提取物）或生长培养基（GM）作为媒介物皮下注射在胁腹中的肿瘤细胞。与GM植入相比，MatrigelTM观察到更高的肿瘤生长速率和细胞密度。组织学还显示，与形成囊肿的GM肿瘤相比，MTG植入细胞作为血管化实体瘤生长。由于采用改进的方法增加了细胞密度，小至0.3 cm 3的肿瘤提供了高S/N的磁共振谱，从而用更少的实验产生更小的标准偏差。
Growth and magnetic resonance characteristics of a human squamous cell carcinoma SQ20B were studiedin vivoas xenografts in nu/nu nude mice. Tumor cells injected subcutaneously in the flank using either MatrigelTM(MTG, an extract of basement membrane proteins) or growth medium (GM) as a vehicle were compared. Much higher tumor growth rates and cell density were observed with MatrigelTMthan with GM implantation. Histology also showed that MTG implanted cells grew as vascularized solid tumors compared to GM tumors which formed cysts. As a result of increased cell density with the improved method, tumors as small as 0.3 cm3provide high S/N magnetic resonance spectra which yield smaller standard deviations with fewer experiments.