THE DNA-BINDING DOMAIN OF THE RAT-LIVER NUCLEAR PROTEIN-C/EBP IS BIPARTITE
THE DNA-BINDING DOMAIN OF THE RAT-LIVER NUCLEAR PROTEIN-C/EBP IS BIPARTITE
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DOI：
10.1126/science.2494700
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发表时间：
1989-03-31
期刊：
影响因子：
56.9
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
--
作者：
LANDSCHULZ, WH;JOHNSON, PF;MCKNIGHT, SL
C/EBP 是一种大鼠肝核蛋白，能够与 DNA 进行序列特异性相互作用。 C/EBP 在体外结合的 DNA 序列与信使 RNA 合成的控制有关。因此预计C/EBP将在调节哺乳动物细胞的基因表达中发挥作用。与 DNA 直接相互作用所需的 C/EBP 多肽区域已被鉴定，并显示其氨基酸序列与 themyc、fos 和 jun 原癌基因的产物相关。这些相关氨基酸序列的排列导致了对一个新结构基序的预测，称为“亮氨酸拉链”，它在促进蛋白质和 DNA 之间的序列特异性相互作用中发挥作用。实验测试现在为亮氨酸拉链假说提供了支持。
C/EBP is a rat liver nuclear protein capable of sequence-specific interaction with DNA. The DNA sequences to which C/EBP binds in vitro have been implicated in the control of messenger RNA synthesis. It has therefore been predicted that C/EBP will play a role in regulating gene expression in mammalian cells. The region of the C/EBP polypeptide required for direct interaction with DNA has been identified and shown to bear amino acid sequence relatedness with the product of themyc,fos, andjunproto-oncogenes. The arrangement of these related amino acid sequences led to the prediction of a new structural motif, termed the "leucine zipper," that plays a role in facilitating sequence-specific interaction between protein and DNA. Experimental tests now provide support for the leucine zipper hypothesis.