Multichannel brain recordings in behaving Drosophila reveal oscillatory activity and local coherence in response to sensory stimulation and circuit activation.
Multichannel brain recordings in behaving Drosophila reveal oscillatory activity and local coherence in response to sensory stimulation and circuit activation.
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DOI：
10.1152/jn.00414.2013
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发表时间：
2013-10
影响因子：
2.5
通讯作者：
A. Paulk;Yanqiong Zhou;Peter G. Stratton;Li Liu;B. van Swinderen
中科院分区：
文献类型：
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作者：
A. Paulk;Yanqiong Zhou;Peter G. Stratton;Li Liu;B. van Swinderen
脊椎动物的神经网络表现出与从感觉处理到运动的功能相关的内源性振荡。目前还不清楚振荡是否在昆虫大脑中发挥类似的作用。我们描述了一种新的“全脑”读出果蝇使用一个简单的多通道记录准备研究电活动的苍蝇暴露于不同的感官刺激的大脑。我们记录了超过200只野生型和转基因动物中超过2，000个注册记录位点的局部场电位（LFP）活动，以揭示与以下相关的特定LFP频带：1）脑区; 2）感觉模态（嗅觉，视觉或机械感觉）;和3）特定神经回路中的活动。我们发现整个苍蝇大脑的内源性和刺激特异性振荡。中央（高阶）大脑区域表现出特定的感官模态增加功率窄频带内。相反，在感觉脑区域，如视叶或触角叶，LFP的连贯性，而不是权力，最好的定义跨模态的感觉反应。通过表达TrpA 1瞬时激活特定回路，我们发现苍蝇大脑中的几个回路调节LFP功率和跨大脑区域和频域的连贯性。然而，神经调节性章鱼胺能回路的激活在视觉刺激期间特异性地增加了视叶中的神经元连贯性，同时降低了中枢脑区域中的连贯性。我们的多通道记录和大脑配准方法提供了一种有效的方法来跟踪活动，同时在体内的苍蝇大脑，允许调查的功能作用，在处理感官刺激和调制行为的振荡。
Neural networks in vertebrates exhibit endogenous oscillations that have been associated with functions ranging from sensory processing to locomotion. It remains unclear whether oscillations may play a similar role in the insect brain. We describe a novel "whole brain" readout for Drosophila melanogaster using a simple multichannel recording preparation to study electrical activity across the brain of flies exposed to different sensory stimuli. We recorded local field potential (LFP) activity from >2,000 registered recording sites across the fly brain in >200 wild-type and transgenic animals to uncover specific LFP frequency bands that correlate with: 1) brain region; 2) sensory modality (olfactory, visual, or mechanosensory); and 3) activity in specific neural circuits. We found endogenous and stimulus-specific oscillations throughout the fly brain. Central (higher-order) brain regions exhibited sensory modality-specific increases in power within narrow frequency bands. Conversely, in sensory brain regions such as the optic or antennal lobes, LFP coherence, rather than power, best defined sensory responses across modalities. By transiently activating specific circuits via expression of TrpA1, we found that several circuits in the fly brain modulate LFP power and coherence across brain regions and frequency domains. However, activation of a neuromodulatory octopaminergic circuit specifically increased neuronal coherence in the optic lobes during visual stimulation while decreasing coherence in central brain regions. Our multichannel recording and brain registration approach provides an effective way to track activity simultaneously across the fly brain in vivo, allowing investigation of functional roles for oscillations in processing sensory stimuli and modulating behavior.