The Role of SDF-1α-CXCR4/CXCR7 in Migration of Human Periodontal Ligament Stem Cells.
The Role of SDF-1α-CXCR4/CXCR7 in Migration of Human Periodontal Ligament Stem Cells.
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DOI：
10.15283/ijsc22053
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发表时间：
2023-05-30
影响因子：
2.3
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中科院分区：
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再生牙髓手术（REP）是牙髓领域的研究热点。 REPs的最大问题之一是难以实现牙髓-牙本质复合体的再生和功能重建。原因尚不清楚。我们假设不同的牙干细胞的迁移可能不同。牙周膜干细胞（PDLSC）可能比根尖乳头干细胞（SCAP）迁移得更快，分化为牙骨质样组织、骨样组织和牙周膜样组织，最终影响 REP 的结果。因此，本研究旨在探讨调节PDLSC迁移的机制。从人第三磨牙中分离培养PDLSC和SCAP后，我们比较了PDLSC和SCAP的迁移情况。然后我们研究了SDF-1α-CXCR4/CXCR7轴在PDLSC迁移中的作用。我们进一步研究了牙龈卟啉单胞菌（P.gingivalis）脂多糖（LPS）对PDLSC迁移的影响及其潜在机制。 PDLSC 在非炎症和炎症条件下均表现出比 SCAP 更好的迁移能力。 SDF-1α可以通过升高CXCR4和CXCR7的表达、增加它们之间的相互作用、促进β-arrestin1的表达以及激活ERK信号通路来促进PDLSC的迁移。 P.gingivalis LPS可通过NF-κB信号通路增加CXCR4的表达、促进β-arrestin1的表达、激活ERK信号通路，从而促进PDLSC向SDF-1α迁移。这项研究有助于阐明牙髓-牙本质复合物难以形成的潜在原因。
Regenerative endodontic procedures (REPs) are a research hotspot in the endodontic field. One of the biggest problems of REPs is that it is difficult to realize regeneration of pulp-dentin complex and functional reconstruction. The reason is still not clear. We hypothesize that the migration may be different in different dental stem cells. Periodontal ligament stem cells (PDLSCs) may migrate faster than stem cells of apical papilla (SCAPs), differentiating into cementum-like tissue, bone-like tissue and periodontal ligament-like tissue and, finally affecting the outcomes of REPs. Hence, this study aimed to explore the mechanism that regulates the migration of PDLSCs. After isolating and culturing PDLSCs and SCAPs from human third molars, we compared the migration of PDLSCs and SCAPs. Then we investigated the role of SDF-1α-CXCR4/CXCR7 axis in PDLSC migration. We further investigated the impact of Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) on PDLSC migration and the potential mechanism. PDLSCs showed better migration under both noninflammatory and inflammatory conditions than SCAPs. SDF-1α can promote the migration of PDLSCs by elevating the expression of CXCR4 and CXCR7, increasing the interaction between them, promoting expression of β-arrestin1 and activating the ERK signaling pathway. P. gingivalis LPS can promote the migration of PDLSCs toward SDF-1α through increasing the expression of CXCR4 via the NF-κB signaling pathway, promoting the expression of β-arrestin1, and activating the ERK signaling pathway. This study helped elucidate the potential reason for the difficulty in forming pulp-dentin complex.