Effects of Endogenous Hormones and Sugars on Fruit Size Driven by Cell Division between Korla Fragrant Pear and Its Bud Mutation
Effects of Endogenous Hormones and Sugars on Fruit Size Driven by Cell Division between Korla Fragrant Pear and Its Bud Mutation
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内源激素和糖对库尔勒香梨及其芽突变细胞分裂驱动果实大小的影响
DOI：
10.21273/hortsci15734-21
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发表时间：
2021-06
期刊：
影响因子：
1.9
通讯作者：
Li Wensheng
中科院分区：
文献类型：
--
作者：
Tian Jia;Wen Yue;Zhang Feng;Sai Jingyi;Zhang Yan;Li Wensheng
大果芽突变是果树育种的重要因素。然而，人们对品种和芽突变之间的差异知之甚少。对库尔勒香梨及其大芽变早美香梨的倍性鉴定表明，其大果性状不是由染色体加倍引起的。对不同时期的中果皮细胞进行计数，发现授粉后中果皮细胞数不断增加，盛花后28 d时差异最大，约为9.4 × 106。28 d后，细胞体积的差异越来越大，细胞体积和细胞数量都是造成库尔勒香梨和早美香梨果实大小差异的原因。为了更深入地了解由细胞分裂驱动的果实大小的差异，我们分析了内源激素[吲哚乙酸（IAA），玉米素核苷（ZR），赤霉素（GA）和脱落酸（阿坝）]和主要糖（葡萄糖，果糖，蔗糖和山梨醇）。早美香梨ZR含量在各生育期均高于库尔勒香梨。细胞分裂过程中阿坝含量除7日龄外，其余时间均相反，差异最大的是28日龄，为30.87 ng/g。阿坝和ZR与细胞数呈负相关。早美香梨在7天后的IAA/ABA、ZR/阿坝和GA/ABA比值始终大于库尔勒香梨，而库尔勒香梨在28天后的IAA/ABA、ZR/ABA和GA/ABA比值均大于早美香梨。21 DAFB时葡萄糖含量的差异最大，为4.80 ng/g。在整个细胞分裂过程中积累了大量的山梨醇。葡萄糖和山梨醇与细胞数呈正相关。综上所述，库尔勒香梨和早美香梨果实中葡萄糖、山梨醇、ZR、阿坝含量及内源激素比例的差异可能与细胞分裂有关。研究结果为库尔勒香梨大果优质生产及芽变的遗传育种提供了理论依据。
Large-fruit bud mutations are important factors in fruit tree breeding. However, little is known about the differences between varieties and bud mutations. The ploidy identification of Korla fragrant pear (Pyrus sinkiangensis Yu) and its large bud mutation Zaomeixiang pear showed that the large-fruit characteristic was not caused by chromosome doubling. By counting mesocarp cells at different stages, we found that the number of cells increased continuously after pollination, and the difference was the greatest at 28 days after full bloom (DAFB), and was about 9.4 × 106. After 28 days, the difference in cell volume became bigger and bigger, so both the cell volume and cell number caused the difference in fruit size between Korla fragrant pear and Zaomeixiang pear. To obtain more insights into the differences in fruit size driven by cell division, we analyzed the endogenous hormones [indole ascetic acid (IAA), zeatin riboside (ZR), gibberellic acid (GA), and abscisic acid (ABA)], and the main sugars (glucose, fructose, sucrose, and sorbitol). The ZR content of Zaomeixiang pear was always greater than that of Korla fragrant pear at all stages. The ABA content was the opposite except for at 7 DAFB during cell division; the greatest difference was 30.87 ng/g, which appeared at 28 DAFB. ABA and ZR correlated negatively with cell number. After 7 DAFB, the ratio of IAA/ABA, ZR/ABA, and GA/ABA in Zaomeixiang pear was always greater than that for Korla fragrant pear at 28 DAFB. The difference in glucose content at 21 DAFB was the greatest, at 4.80 ng/g. Large amounts of sorbitol accumulated during whole-cell division. Glucose and sorbitol correlated positively with cell numbers. In summary, the data suggest that the different contents of glucose, sorbitol, ZR, and ABA, and the ratio of endogenous hormones might be related to cell division in Korla fragrant pear and Zaomeixiang pear. The result provides a theoretical basis for the large-size fruit’s high-quality production and genetic breeding of Korla fragrant pear and its bud mutation.
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平行芽突变测序揭示果实糖和酸代谢可能影响苹果的应激
影响因子：
5.6
作者：
Zhao Jirong;Shen Fei;Gao Yuan;Wang Dajiang;Wang Kun
通讯作者：
Wang Kun
影响因子：
4.2
作者：
Caixi Zhang;K. Tanabe;Shiping Wang;F. Tamura;Akira Yoshida;Kazuhiro Matsumoto
通讯作者：
Caixi Zhang;K. Tanabe;Shiping Wang;F. Tamura;Akira Yoshida;Kazuhiro Matsumoto
影响因子：
4.1
作者：
Nardozza, Simona;Cooney, Janine;Richardson, Annette C.
通讯作者：
Richardson, Annette C.
影响因子：
--
作者：
S. Zebin
通讯作者：
S. Zebin
DOI：
10.3390/agronomy9010020
发表时间：
2019-01
期刊：
Agronomy
影响因子：
--
作者：
F. B. M. D. Souza;E. Alves;R. Pio;Elisa de Melo Castro;G. Reighard;A. I. Freire;N. Mayer;R. Pimentel
通讯作者：
F. B. M. D. Souza;E. Alves;R. Pio;Elisa de Melo Castro;G. Reighard;A. I. Freire;N. Mayer;R. Pimentel