Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.
Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.
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DOI：
10.1139/g08-013
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发表时间：
2008-04
期刊：
影响因子：
3.1
通讯作者：
Yi Zhang;J. Mao;Kun Yang;Yunfeng Li;Jian Zhang;Yuanxiang Huang;Fucheng Shen;Chaochao Zhang
中科院分区：
文献类型：
--
作者：
Yi Zhang;J. Mao;Kun Yang;Yunfeng Li;Jian Zhang;Yuanxiang Huang;Fucheng Shen;Chaochao Zhang
在恢复系金恢一号的后代中发现了一种自发突变的雄性不育材料。为了了解相关基因的状态和功能，并克隆该基因，选育了一个雄性不育近等基因系(NIL)，并对突变体进行了鉴定和基因定位。结果表明，不育系与保持系II-32B之间存在明显差异。近交系的花药大小小于II-32B，花药颜色为白色，II-32B为黄色。在KI-I2溶液中没有观察到来自近交系成熟花药的花粉。在不育花药的横切面上，在小孢子早期，绒毡层的细胞质集中，但当小孢子从四分体中释放出来后，绒毡层本身并不退化；随后，绒毡层从花药壁脱落并包裹小孢子；随后，被包围的小孢子在小孢子晚期和双细胞花粉早期完全崩溃。遗传分析表明，该不育系由1对隐性基因ostd(T)控制。该基因定位于第6染色体上的SSR标记RM7434和RM275之间，RM7434与RM275之间的物理距离约为389kb。
A spontaneously mutated male-sterile material was found among the offspring of the indica restorer line Jinhuiyihao. To understand the status and function of the related gene and clone the gene, a near-isogenic line (NIL) of the male sterility was bred, and characterization of the mutant and gene mapping were performed. The results indicated that there are obvious differences between the male-sterile NIL and the indica maintainer line II-32B. The anther size of the NIL is smaller than that of II-32B, and the anther color is white in the NIL but yellow in II-32B. No pollen from the matured anther in the NIL was observed to be stained using KI-I2 solution. In transverse sections of the sterile anther, at early microspore stage the cytoplasm of the tapetum concentrates but the tapetum itself does not degenerate after microspores are released from the tetrads; the tapetum then desquamates from the anther wall and enwraps microspores; subsequently, the surrounded microspores collapse completely at late microspore and early bicellular pollen stages. Inheritance analysis showed that the male sterility was controlled by a single recessive gene, ostd (t). This gene was mapped between the SSR markers RM7434 and RM275 on chromosome 6, and the physical distance from RM7434 to RM275 is about 389 kb.