Morphology and localization of interstitial cells in the guinea pig bladder: Structural relationships with smooth muscle and neurons
Morphology and localization of interstitial cells in the guinea pig bladder: Structural relationships with smooth muscle and neurons
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DOI：
10.1097/01.ju.0000146272.80848.37
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发表时间：
2005-04-01
影响因子：
6.6
通讯作者：
McCloskey, KD
中科院分区：
文献类型：
--
作者：
Davidson, RA;McCloskey, KD
目的：在本研究中，我们研究了豚鼠膀胱壁中Cajal间质细胞（ICC）样细胞的位置，并研究了它们与神经和平滑肌细胞的结构相互作用。材料和方法：膀胱组织的整体标本和冷冻切片用一抗和荧光二抗标记，并使用共聚焦和多光子显微镜成像。Kit阳性ICC样细胞位于尿道下段、固有层区域和整个逼尿肌。在内皮下，它们具有星状形态并且似乎呈网络状。它们与神经连接，如用抗kit和抗蛋白质基因产物9.5的双标记实验所示。还发现波形蛋白阳性细胞的网络，其中许多但不是全部是kit阳性的。在逼尿肌中，kit阳性细胞多见于平滑肌束边缘。它们与束平行走行，并与壁内神经紧密相连。在逼尿肌肌束间可见另一群kit阳性细胞。这些细胞具有更像恒星的形态，并相互连接。Kit阳性细胞追踪神经束并靠近壁内神经节。波形蛋白阳性细胞存在于逼尿肌，其中一些也是kit阳性。结论：有几个群体的ICC样细胞遍布豚鼠膀胱壁。它们在形态和方向上不同，但都与壁内神经连接，在逼尿肌中，它们与平滑肌细胞密切相关。
Purpose: In the current study we examined the location of interstitial cell of Cajal (ICC)-like cells in the guinea pig bladder wall and studied their structural interactions with nerves and smooth muscle cells.Materials and Methods: Whole mount samples and cryosections of bladder tissue were labeled with primary and fluorescent secondary antibodies, and imaged using confocal and multiphoton microscopy.Results: Kit positive ICC-like cells were located below the urothelium, in the lamina propria region and throughout the detrusor. In the suburothelium they had a stellate morphology and appeared to network. They made connections with nerves, as shown by double labeling experiments with anti-kit and anti-protein gene product 9.5. A network of vimentin positive cells was also found, of which many but not all were kit positive. In the detrusor kit positive cells were most often seen at the edge of smooth muscle bundles. They were elongated with lateral branches, running in parallel with the bundles and closely associated with intramural nerves. Another population of kit positive cells was seen in the detrusor between muscle bundles. These cells had a more stellate-like morphology and made connections with each other. Kit positive cells were seen tracking nerve bundles and close to intramural ganglia. Vimentin positive cells were present in the detrusor, of which some were also kit positive.Conclusions: There are several populations of ICC-like cells throughout the guinea pig bladder wall. They differ in morphology and orientation but all make connections with intramural nerves and in the detrusor they are closely associated with smooth muscle cells.