INFECTION OF RATS WITH HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 - SUSCEPTIBILITY OF INBRED STRAINS, ANTIBODY-RESPONSE AND PROVIRUS LOCATION
INFECTION OF RATS WITH HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 - SUSCEPTIBILITY OF INBRED STRAINS, ANTIBODY-RESPONSE AND PROVIRUS LOCATION
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DOI：
10.1002/ijc.2910580324
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发表时间：
1994-08-01
影响因子：
6.4
通讯作者：
BOMFORD, R
中科院分区：
文献类型：
--
作者：
IBRAHIM, F;FIETTE, L;BOMFORD, R
比较了不同品系近交系大鼠接种人T细胞白血病病毒（HTLV-Ⅰ）生产细胞系后对HTLV-Ⅰ感染的敏感性。Fisher F344和Brown Norway菌株对HTLV-1产生了最高的抗体应答，而刘易斯和BB菌株是低应答者。用来自HTLV-1感染的Fischer F344和Brown Norway大鼠的血清在Western印迹中检测到抗HTLV-1 gag蛋白和env gp21但未检测到env gp46的抗体。这些血清在体外合胞体形成抑制试验中无活性。通过聚合酶链反应在所有Fischer F344大鼠、部分刘易斯和Brown Norway大鼠中检测到HTLV-Ⅰ前病毒，但在缺乏CD 8（+）T淋巴细胞的BB大鼠中未检测到。在感染后12周，Fischer F344、刘易斯和Brown Norway大鼠中HTLV-1前病毒最常见的位置是外周血单核细胞（PBMC）和脊髓。在Brown Norway大鼠的第二个实验中，在12周时在大鼠的PBMC中再次检测到前病毒，但在22周时未检测到，并且在22周时测试的其他器官中，交感神经节呈阳性。结论是HTLV-I感染发生在成年大鼠，但随着时间的推移被抑制。
The susceptibilities of different strains of inbred rats to infection with the human T-cell leukemia virus (HTLV-I) after inoculation of human HTLV-I producer cell lines were compared. The Fisher F344 and Brown Norway strains developed the highest antibody response to HTLV-I, while the Lewis and BB strains were low responders. Antibodies against the HTLV-I gag proteins, and env gp21 but not env gp46, were detected in Western blots with sera from HTLV-I-infected Fischer F344 and Brown Norway rats. These sera were inactive in an in vitro syncytium-formation inhibition test. The HTLV-I provirus was detected by polymerase chain reaction in all Fischer F344, and some Lewis and Brown Norway rats, but not in the BB, which lack CD8(+) T lymphocytes. The most frequent locations of the HTLV-I provirus in the Fischer F344, Lewis and Brown Norway rats at 12 weeks after infection were the peripheral blood mononuclear cells (PBMC) and spinal cord. In a second experiment in Brown Norway rats, the provirus was again detected in the PBMC of rats at 12 weeks, but not at 22 weeks, and among the other organs tested at 22 weeks the sympathetic nerve ganglia were positive. It is concluded that HTLV-I infection occurs in adult rats, but is suppressed with time.