Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction
Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction
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DOI：
10.1016/j.bios.2004.08.030
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发表时间：
2005-07-15
影响因子：
12.6
通讯作者：
Matsumoto, K
中科院分区：
文献类型：
--
作者：
Takeda, S;Sbagyo, A;Matsumoto, K
碳纳米管传感器检测到抗血凝素结合固定血凝素。需要对血清中的抗体或抗原进行超灵敏检测的方法。将血凝素固定在碳纳米管的背面，从而产生源极和漏极。电极垫覆盖了纳米管的每个边缘。源极和漏极之间的1-V曲线是在纳米管的背面上的缓冲溶液中将抗血凝素与固定的血凝素孵育后测量的。CNT传感器的灵敏度高于ELISA系统。这种方法构成了一种新的工具来分析在基板上的生物分子之间的相互作用。(c)2004 Elsevier B.V.保留所有权利。
Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The 1-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate. (c) 2004 Elsevier B.V. All rights reserved.