IN-VITRO LIPID-SYNTHESIS IN HUMAN ADIPOSE-TISSUE FROM 3 ABDOMINAL SITES
IN-VITRO LIPID-SYNTHESIS IN HUMAN ADIPOSE-TISSUE FROM 3 ABDOMINAL SITES
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DOI：
10.1152/ajpendo.1993.265.3.e374
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发表时间：
1993-09-01
影响因子：
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通讯作者：
LEIBEL, RL
中科院分区：
文献类型：
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作者：
EDENS, NK;FRIED, SK;LEIBEL, RL
腹部脂肪组织的沉积与肥胖的发病率之间的关联可能与腹部扩大的脂肪储存物释放的高游离脂肪酸有关。为了探讨可能导致腹内脂肪细胞增大的细胞机制，对严重肥胖的女性和男性腹部皮下、大网膜和肠系膜脂肪组织进行了[C-14]葡萄糖的脂肪分解、三酰甘油(TG)和二酰甘油(DG)合成的测定。与腹部或男性的任何部位相比，女性皮下脂肪组织的甘油三酯合成率最高。与皮下脂肪组织相比，异丙肾上腺素更能刺激腹内脂肪组织中TG的合成。在基础状态下，男性和女性腹内脂肪组织中的[C-14]DG积累率约为总[C-14]甘油三酯积累率的50%，而皮下脂肪组织中的[C-14]DG积累率约为总积累量的25%。腹内脂肪细胞的基础脂肪分解低于皮下脂肪细胞。刺激脂肪分解可比皮下脂肪组织更多地减少[C-14]DG在腹部组织中的蓄积。在腹部脂肪细胞中，低速率的甘油三酯合成率可能会阻止女性体内大量脂肪的蓄积。
The association between abdominal deposition of adipose tissue and morbidities accompanying obesity may be related to high rates of free fatty acid release from enlarged intra-abdominal stores. To investigate cellular mechanisms that might contribute to enlargement of intra-abdominal adipocytes, lipolysis, triacylglycerol (TG), and diacylglycerol (DG) synthesis from [C-14] glucose was measured in abdominal subcutaneous, omental, and mesenteric adipose tissue from severely obese women and men. Subcutaneous adipose tissue from women showed the highest rates of TG synthesis compared with the intra-abdominal site, or any site in men. Isoproterenol stimulated TG synthesis more in intra-abdominal than subcutaneous adipose tissue. In the basal state, intra-abdominal adipose tissue from both men and women showed rates of [C-14]DG accumulation approximately 50% total [C-14]acylglyceride accumulation, whereas, in subcutaneous adipose tissue, [C-14]DG accumulation was approximately 25% of total. Basal lipolysis was lower in intra-abdominal than subcutaneous adipocytes. Stimulation of lipolysis reduced [C-14]DG accumulation more in intra-abdominal than subcutaneous adipose tissue. Low rates of acylglyceride synthesis in intra-abdominal adipocytes may prevent accumulation of large intra-abdominal fat stores in women.