IRON CHELATION FOR AMELIORATION OF LIVER ISCHEMIA-REPERFUSION INJURY
IRON CHELATION FOR AMELIORATION OF LIVER ISCHEMIA-REPERFUSION INJURY
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DOI：
10.3109/03630269.2010.484766
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发表时间：
2010-01-01
期刊：
影响因子：
1
通讯作者：
Smyrniotis, Vassilios
中科院分区：
文献类型：
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作者：
Arkadopoulos, Nikolaos;Nastos, Constantinos;Smyrniotis, Vassilios
肝切除术经常与肝残体的显著缺血-再灌注（I-R）损伤相关。本研究的目的是研究去铁胺（DFO）是否可以改善在猪模型中进行的主要肝切除术在肝脏血管阻断下的I-R损伤。12只雌性家猪分为对照组（n = 6）和DFO治疗组（n = 6），进行150分钟的肝缺血，然后进行70%肝切除和24小时再灌注。DFO组连续静脉输注100 mg/kg DFO。通过肝功能检查、肝组织病理学检查以及血清和肝组织丙二醛（MDA）浓度评价肝残余损伤。去铁胺处理的动物具有降低的总胆红素、γ-谷氨酰转移酶和氨水平以及肝细胞坏死和氧化损伤。在随后的随机临床试验中，使用DFO在主要肝脏手术期间进行I-R保护，初步结果显示肝细胞损伤、氧化和炎症血清标志物以及肝残端活检中的凋亡反应得到改善。
Liver resections are frequently associated with significant ischemia-reperfusion (I-R) injury of the liver remnant. The aim of this study was to investigate whether deferoxamine (DFO) can ameliorate I-R injury during major hepatectomies performed under vascular exclusion of the liver in a porcine model. Twelve female domestic pigs were divided into control (n = 6) and DFO treatment (n = 6) groups and subjected to 150 min. liver ischemia followed by 70% hepatectomy and 24 hours reperfusion. Pigs in the DFO group received a continuous intravenous infusion of 100 mg/kg DFO. Liver remnant injury was evaluated by liver function tests, hepatic histology as well as serum and liver tissue malondialdehyde (MDA) concentrations. Deferoxamine-treated animals had reduced total bilirubin,gamma-glutamyl transferase and ammonia levels as well as hepatocyte necrosis and oxidative injury. In a subsequent randomized clinical trial using DFO for I-R protection during major liver surgery, preliminary results revealed amelioration of hepatocellular damage, oxidative and inflammatory serum markers and apoptotic response in liver remnant biopsies.