Multiplex Immunofluorescence Assays
Multiplex Immunofluorescence Assays
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DOI：
10.1007/978-1-4939-9773-2_22
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发表时间：
2020-01-01
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影响因子：
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通讯作者：
Wistuba, Ignacio I.
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作者：
Francisco-Cruz, Alejandro;Parra, Edwin Roger;Wistuba, Ignacio I.
近年来，同时检测同一组织切片中多个表位的多路成像平台成为研究肿瘤免疫结构的非常强大的工具。这些革命性的技术为福尔马林固定和石蜡包埋（FFPE）的肿瘤评估提供了一种深入的方法，以提高对肿瘤微环境、治疗新靶点、预后和预测生物标志物以及转化研究的理解。多重成像平台允许从单个组织切片、芯针活检和组织微阵列同时鉴定几种抗原。近年来，多重成像提高了表征恶性和非恶性组织中不同类型的细胞群及其与临床结果相关的空间分布的能力。与数字图像分析软件相关的多路复用技术提供了高质量的通量测定，以在治疗前、治疗期间和治疗后的多个时间点研究癌症标本。本章的目的是提供一个审查的多重成像，涵盖其基本原理，优点，缺点，材料和方法，适用于FFPE肿瘤组织染色，重点是使用多重免疫荧光与酪胺信号放大染色的免疫分析和翻译研究。
Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue section emerged in the last years as very powerful tools to study tumor immune contexture. These revolutionary technologies are providing a deep methodology for tumor evaluation in formalin-fixed and paraffin-embedded (FFPE) to improve the understanding of tumor microenvironment, new targets for treatment, prognostic and predictive biomarkers, and translational studies. Multiplexed imaging platforms allow for the identification of several antigens simultaneously from a single tissue section, core needle biopsies, and tissue microarrays. In recent years, multiplexed imaging has improved the abilities to characterize the different types of cell populations in malignant and non-malignant tissues, and their spatial distribution in relationship to clinical outcomes. Multiplexed technologies associated with digital image analysis software offer a high-quality throughput assay to study cancer specimens at multiple time points before, during and after treatment. The aim of this chapter is to provide a review of multiplexed imaging covering its fundamentals, advantages, disadvantages, and material and methods for staining applied to FFPE tumor tissues and focusing on the use of multiplex immunofluorescence with tyramine signal amplification staining for immune profiling and translational research.